Structural role of essential light chains in the apicomplexan glideosome.

Pazicky S Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C, Commun Biol 3(1):568 (2020) Europe PMC

SASDH84 – Toxoplasma gondii myosin essential light chain 2

Myosin essential light chain 2
MWI(0) 17 kDa
MWexpected 15 kDa
VPorod 27 nm3
log I(s) 1.32×10-2 1.32×10-3 1.32×10-4 1.32×10-5
Myosin essential light chain 2 small angle scattering data  s, nm-1
ln I(s)
Myosin essential light chain 2 Guinier plot ln 1.32×10-2 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Myosin essential light chain 2 Kratky plot 1.104 0 3 sRg
p(r)
Myosin essential light chain 2 pair distance distribution function Rg: 2.1 nm 0 Dmax: 6.7 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Toxoplasma gondii myosin essential light chain 2 in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20.1°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Myosin essential light chain 2
Mol. type   Protein
Organism   Toxoplasma gondii
Olig. state   Monomer
Mon. MW   15.5 kDa
 
UniProt   B9PZ33 (1-133)
Sequence   FASTA