Structural role of essential light chains in the apicomplexan glideosome.

Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
Commun Biol 3(1):568 (2020 Oct 13)

PMID: 33051581 doi: 10.1038/s42003-020-01283-8

Submitted to SASBDB: 2019 Dec 19 Published in SASBDB: 2020 Oct 26

SASDH64 – Plasmodium falciparum myosin essential light chain, N-terminal domain Sample: Myosin essential light chain monomer, 9 kDa Plasmodium falciparum protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Oct 25 RgGuinier 1.4 nm Dmax 4.3 nm VolumePorod 12 nm3

SASDH74 – Plasmodium falciparum myosin essential light chain, full-length Sample: Myosin essential light chain monomer, 16 kDa Plasmodium falciparum protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jun 30 RgGuinier 2.7 nm Dmax 9.5 nm VolumePorod 23 nm3

SASDH84 – Toxoplasma gondii myosin essential light chain 2 Sample: Myosin essential light chain 2 monomer, 15 kDa Toxoplasma gondii protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 8 RgGuinier 2.1 nm Dmax 6.7 nm VolumePorod 27 nm3

SASDH94 – Toxoplasma gondii myosin essential light chain 2 (TgELC2) bound to myosin A C-terminus Sample: Myosin essential light chain 2 monomer, 15 kDa Toxoplasma gondii protein Myosin A monomer, 3 kDa Toxoplasma gondii protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 8 RgGuinier 1.7 nm Dmax 5.5 nm VolumePorod 28 nm3

SASDHA4 – Trimeric complex of myosin A with myosin light chain (MLC1) and essential light chain (TgELC1) from Toxoplasma gondii Sample: Toxoplasma gondii essential light chain 1 monomer, 15 kDa Toxoplasma gondii protein Myosin A monomer, 5 kDa Toxoplasma gondii protein Toxoplasma gondii myosin light chain, full length monomer, 24 kDa Toxoplasma gondii protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30 RgGuinier 3.2 nm Dmax 14.0 nm VolumePorod 69 nm3

SASDHB4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 66-210) and essential light chain (TgELC1) from Toxoplasma gondii Sample: Myosin light chain TgMLC1, residues 66-210 monomer, 17 kDa Toxoplasma gondii protein Toxoplasma gondii essential light chain 1 monomer, 15 kDa Toxoplasma gondii protein Myosin A monomer, 5 kDa Toxoplasma gondii protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30 RgGuinier 2.7 nm Dmax 9.5 nm VolumePorod 50 nm3

SASDHC4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 70-210) and essential light chain (TgELC1) from Toxoplasma gondii Sample: Toxoplasma gondii essential light chain 1 monomer, 15 kDa Toxoplasma gondii protein Myosin A monomer, 5 kDa Toxoplasma gondii protein Toxoplasma gondii myosin light chain, residues 70-210 monomer, 17 kDa Toxoplasma gondii protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30 RgGuinier 2.5 nm Dmax 8.2 nm VolumePorod 47 nm3

SASDHD4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 66-210) and essential light chain (TgELC2) from Toxoplasma gondii Sample: Myosin essential light chain 2 monomer, 15 kDa Toxoplasma gondii protein Myosin light chain TgMLC1, residues 66-210 monomer, 17 kDa Toxoplasma gondii protein Myosin A monomer, 5 kDa Toxoplasma gondii protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30 RgGuinier 2.7 nm Dmax 10.0 nm VolumePorod 50 nm3

SASDHE4 – Trimeric complex of myosin A with myosin tail interacting protein (MTIP, residues 60-204) and essential light chain (PfELC) from Plasmodium falciparum Sample: Myosin essential light chain monomer, 16 kDa Plasmodium falciparum protein Plasmodium falciparum myosin A monomer, 5 kDa Plasmodium falciparum protein Myosin A tail domain interacting protein monomer, 17 kDa Plasmodium falciparum protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30 RgGuinier 2.7 nm Dmax 10.7 nm VolumePorod 51 nm3