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12 hits found for Pazicky

SASDH64 – Plasmodium falciparum myosin essential light chain, N-terminal domain

Myosin essential light chain experimental SAS data
PYMOL model
Sample: Myosin essential light chain monomer, 9 kDa Plasmodium falciparum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Oct 25
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 1.4 nm
Dmax 4.3 nm
VolumePorod 12 nm3

SASDH74 – Plasmodium falciparum myosin essential light chain, full-length

Myosin essential light chain experimental SAS data
Myosin essential light chain Kratky plot
Sample: Myosin essential light chain monomer, 16 kDa Plasmodium falciparum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jun 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 23 nm3

SASDH84 – Toxoplasma gondii myosin essential light chain 2

Myosin essential light chain 2 experimental SAS data
Myosin essential light chain 2 Kratky plot
Sample: Myosin essential light chain 2 monomer, 15 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 8
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.1 nm
Dmax 6.7 nm
VolumePorod 27 nm3

SASDH94 – Toxoplasma gondii myosin essential light chain 2 (TgELC2) bound to myosin A C-terminus

Myosin essential light chain 2Myosin A experimental SAS data
PYMOL model
Sample: Myosin essential light chain 2 monomer, 15 kDa Toxoplasma gondii protein
Myosin A monomer, 3 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 8
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 1.7 nm
Dmax 5.5 nm
VolumePorod 28 nm3

SASDHA4 – Trimeric complex of myosin A with myosin light chain (MLC1) and essential light chain (TgELC1) from Toxoplasma gondii

Toxoplasma gondii essential light chain 1Myosin AToxoplasma gondii myosin light chain, full length experimental SAS data
PYMOL model
Sample: Toxoplasma gondii essential light chain 1 monomer, 15 kDa Toxoplasma gondii protein
Myosin A monomer, 5 kDa Toxoplasma gondii protein
Toxoplasma gondii myosin light chain, full length monomer, 24 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 3.2 nm
Dmax 14.0 nm
VolumePorod 69 nm3

SASDHB4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 66-210) and essential light chain (TgELC1) from Toxoplasma gondii

Myosin light chain TgMLC1, residues 66-210Toxoplasma gondii essential light chain 1Myosin A experimental SAS data
PYMOL model
Sample: Myosin light chain TgMLC1, residues 66-210 monomer, 17 kDa Toxoplasma gondii protein
Toxoplasma gondii essential light chain 1 monomer, 15 kDa Toxoplasma gondii protein
Myosin A monomer, 5 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 50 nm3

SASDHC4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 70-210) and essential light chain (TgELC1) from Toxoplasma gondii

Toxoplasma gondii essential light chain 1Myosin AToxoplasma gondii myosin light chain, residues 70-210 experimental SAS data
PYMOL model
Sample: Toxoplasma gondii essential light chain 1 monomer, 15 kDa Toxoplasma gondii protein
Myosin A monomer, 5 kDa Toxoplasma gondii protein
Toxoplasma gondii myosin light chain, residues 70-210 monomer, 17 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 47 nm3

SASDHD4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 66-210) and essential light chain (TgELC2) from Toxoplasma gondii

Myosin essential light chain 2Myosin light chain TgMLC1, residues 66-210Myosin A experimental SAS data
PYMOL model
Sample: Myosin essential light chain 2 monomer, 15 kDa Toxoplasma gondii protein
Myosin light chain TgMLC1, residues 66-210 monomer, 17 kDa Toxoplasma gondii protein
Myosin A monomer, 5 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 50 nm3

SASDHE4 – Trimeric complex of myosin A with myosin tail interacting protein (MTIP, residues 60-204) and essential light chain (PfELC) from Plasmodium falciparum

Myosin essential light chainPlasmodium falciparum myosin AMyosin A tail domain interacting protein experimental SAS data
Myosin essential light chain Plasmodium falciparum myosin A Myosin A tail domain interacting protein Kratky plot
Sample: Myosin essential light chain monomer, 16 kDa Plasmodium falciparum protein
Plasmodium falciparum myosin A monomer, 5 kDa Plasmodium falciparum protein
Myosin A tail domain interacting protein monomer, 17 kDa Plasmodium falciparum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.7 nm
Dmax 10.7 nm
VolumePorod 51 nm3

SASDJF4 – Synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23 experimental SAS data
CORAL model
Sample: Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein
Buffer: 50 mM Tris 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 May 5
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 Nature Communications 11(1) (2020)
...Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 22 nm3

SASDJG4 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD)

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data
SASREF model
Sample: Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer: 25 mM Tris 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 May 1
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 Nature Communications 11(1) (2020)
...Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C
RgGuinier 3.0 nm
Dmax 13.1 nm
VolumePorod 64 nm3

SASDJH4 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) bound to the synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data
CORAL model
Sample: Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein
Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer: 25 mM Tris 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 May 10
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 Nature Communications 11(1) (2020)
...Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C
RgGuinier 3.5 nm
Dmax 15.1 nm
VolumePorod 87 nm3