Hyperstable De Novo Protein with a Dimeric Bisecting Topology.

Kimura N, Mochizuki K, Umezawa K, Hecht MH, Arai R, ACS Synth Biol (2020) Europe PMC

SASDHW3 – Binary-patterned 4-helix bundle de novo protein WA20

De novo protein WA20
MWI(0) 24 kDa
MWexpected 25 kDa
log I(s) 1.90×10-3 1.90×10-4 1.90×10-5 1.90×10-6
De novo protein WA20 small angle scattering data  s, nm-1
ln I(s)
De novo protein WA20 Guinier plot ln 1.90×10-3 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
De novo protein WA20 Kratky plot 1.104 0 √3 sRg
p(r)
De novo protein WA20 pair distance distribution function Rg: 2.6 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
De novo protein WA20 PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
De novo protein WA20 DAMMIN model
Synchrotron SAXS data from solutions of Binary-patterned 4-helix bundle de novo protein WA20 in 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH 7.5 were collected on the BL-6A beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring (Tsukuba, Japan) using a Pilatus3 1M detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 30 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

De novo protein WA20 (WA20)
Mol. type   Protein
Organism   de novo protein
Olig. state   Dimer
Mon. MW   12.5 kDa
Sequence   FASTA
 
PDB ID   3VJF