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7 hits found for De novo protein WA20

SASDHW3 – ...de novo protein WA20

De novo protein WA20 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: De novo protein WA20 dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Feb 26
...De Novo Protein with a Dimeric Bisecting Topology. ACS Synth Biol (2020)
Kimura N, Mochizuki K, Umezawa K, Hecht MH, Arai R
RgGuinier 2.7 nm
Dmax 10.0 nm

SASDHX3 – ...de novo protein Super WA20 (SUWA)

Hyperstable de novo protein Super WA20 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...de novo protein Super WA20 dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Feb 26
...De Novo Protein with a Dimeric Bisecting Topology. ACS Synth Biol (2020)
Kimura N, Mochizuki K, Umezawa K, Hecht MH, Arai R
RgGuinier 2.7 nm
Dmax 10.0 nm

SASDD46 – ...designed de novo protein esPN-Block (HL4) heterocomplex, e1s2 (HL4)

extender PN-Block (HL4)stopper PN-Block (WA20) experimental SAS data
DAMMIN model
Sample: ...der PN-Block (HL4) monomer, 27 kDa de novo protein protein
...WA20) dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on ...Dec 19
...de Novo Extender Protein Nanobuilding Blocks. ACS Synth Biol 7(5):1381-1394 (2018)
Kobayashi N, Inano K, Sasahara K, Sato T, Miyazawa K, Fukuma T, Hecht MH, Song C, Murata K, Arai R
RgGuinier 3.6 nm
Dmax 15.0 nm

SASDD56 – ...designed de novo protein esPN-Block (FL4) heterocomplex, e1s2 (FL4)

stopper PN-Block (WA20)extender PN-Block (FL4) experimental SAS data
DAMMIN model
Sample: ...WA20) dimer, 25 kDa de novo protein protein
...der PN-Block (FL4) monomer, 27 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on ...Dec 19
...de Novo Extender Protein Nanobuilding Blocks. ACS Synth Biol 7(5):1381-1394 (2018)
Kobayashi N, Inano K, Sasahara K, Sato T, Miyazawa K, Fukuma T, Hecht MH, Song C, Murata K, Arai R
RgGuinier 3.3 nm
Dmax 12.0 nm

SASDKM8 – ...WA20 (ROWA) dimer

Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer experimental SAS data
DAMMIN model
Sample: ...WA20 mutant N22A/H86K (ROWA) dimer dimer, 25 kDa ...protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
...de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 2.5 nm
Dmax 9.9 nm
VolumePorod 30 nm3

SASDKN8 – ...WA20 (ROWA) tetramer

Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer experimental SAS data
DAMMIN model
Sample: ...WA20 mutant N22A/H86K (ROWA) tetramer tetramer, 50 kDa ...protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
...de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 4.1 nm
Dmax 18.1 nm
VolumePorod 60 nm3

SASDKP8 – ...WA20 (ROWA) hexamer

Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer experimental SAS data
DAMMIN model
Sample: ...WA20 mutant N22A/H86K (ROWA) hexamer hexamer, 75 kDa ...protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
...de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 5.3 nm
Dmax 24.8 nm
VolumePorod 120 nm3