Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.

Tamulaitiene G, Jovaisaite V, Tamulaitis G, Songailiene I, Manakova E, Zaremba M, Grazulis S, Xu SY, Siksnys V, Nucleic Acids Res 45(6):3547-3558 (2017) Europe PMC

SASDJB2 – Restriction endonuclease R.AgeI in apo form, monomeric

Type-2 restriction enzyme AgeI
MWI(0) 27 kDa
MWexpected 31 kDa
VPorod 53 nm3
log I(s) 2.57×10-2 2.57×10-3 2.57×10-4 2.57×10-5
Type-2 restriction enzyme AgeI small angle scattering data  s, nm-1
ln I(s)
Type-2 restriction enzyme AgeI Guinier plot ln 2.57×10-2 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
Type-2 restriction enzyme AgeI Kratky plot 1.104 0 3 sRg
p(r)
Type-2 restriction enzyme AgeI pair distance distribution function Rg: 2.1 nm 0 Dmax: 6.7 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Type-2 restriction enzyme AgeI DAMMIN model

log I(s)
 s, nm-1
Type-2 restriction enzyme AgeI PDB model

Synchrotron SAXS data from solutions of apo-R.AgeI in 10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl₂ were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.2 and 3.1 mg/ml were measured at 20°C. Eight successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Type-2 restriction enzyme AgeI (R.AgeI)
Mol. type   Protein
Organism   Thalassobius gelatinovorus
Olig. state   Monomer
Mon. MW   30.5 kDa
 
UniProt   Q9KHV6 (1-278)
Sequence   FASTA
 
PDB code   5DWC