Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.

Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K, Sci Adv 7(22) (2021) Europe PMC

SASDJJ6 – Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1)

N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2)
MWI(0) 26 kDa
MWexpected 20 kDa
VPorod 46 nm3
log I(s) 8.20×10-3 8.20×10-4 8.20×10-5 8.20×10-6
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) small angle scattering data  s, nm-1
ln I(s)
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) Guinier plot ln 8.20×10-3 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) Kratky plot 1.104 0 3 sRg
p(r)
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) pair distance distribution function Rg: 3.7 nm 0 Dmax: 14.1 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1) Rg histogram Rg, nm
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model

Synchrotron SAXS data from solutions of N-FATZ-1 in 50 mM Tris-HCl 150 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl of sample was injected at a 0.30 ml/min flow rate onto a GE Superose 6 Increase 5/150 column at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Three SEC-SAXS runs were averaged to generate the dataset displayed in this entry. The three individual and averaged SEC-SAXS profiles (obtained from 67-100 0.25 s exposures through the major SEC-elution peak using either 4.7 or 2.3 mg/ml sample injection concentrations) are included in the full entry zip archive. The MW estimate from parallel MALLS-RI measurements is 22 kDa.

N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) (N-FATZ-1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   19.6 kDa
 
UniProt   Q9NP98 (1-174)
Sequence   FASTA