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12 hits found for Histidine kinase

SASDHY3 – KinA-Sda complex

Sporulation kinase ASporulation inhibitor sda experimental SAS data
SASREF model
Sample: ...kinase A dimer, 51 kDa Bacillus subtilis protein
Sporulation inhibitor sda dimer, 11 kDa Bacillus subtilis protein
Buffer: 50mM Tris, 200mM NaCl, 150mM Imidazole, pH: 8.5
Experiment: SAXS data collected at Bruker Nanostar II, Australian Nuclear Science and Technology Organisation/Australian Centre for Neutron Scattering on 2006 Nov 16
...histidine kinase inhibition. J Mol Biol 368(2):407-20 (2007)
Whitten AE, Jacques DA, Hammouda B, Hanley T, King GF, Guss JM, Trewhella J, Langley DB
RgGuinier 2.9 nm
Dmax 8.0 nm
VolumePorod 85 nm3

SASDHZ3 – ...kinase A

Sporulation kinase A experimental SAS data
SASREF model
Sample: ...kinase A dimer, 51 kDa Bacillus subtilis protein
Buffer: 50mM Tris, 200mM NaCl, 150mM Imidazole, pH: 8.5
Experiment: SAXS data collected at Bruker Nanostar II, Australian Nuclear Science and Technology Organisation/Australian Centre for Neutron Scattering on 2006 Mar 18
...histidine kinase inhibition. J Mol Biol 368(2):407-20 (2007)
Whitten AE, Jacques DA, Hammouda B, Hanley T, King GF, Guss JM, Trewhella J, Langley DB
RgGuinier 2.9 nm
Dmax 9.5 nm
VolumePorod 76 nm3

SASDAA7 – ...histidine protein kinase and response regulator

Histidine protein kinaseResponse regulator experimental SAS data
BUNCH model
Sample: Histidine protein kinase dimer, 54 kDa Streptococcus pneumoniae protein
Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 200 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2012 May 16
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S
RgGuinier 4.0 nm
Dmax 16.0 nm
VolumePorod 175 nm3

SASDDH7 – ...histidine kinase, CitA, fused to lipase EstA in the presence of citrate

citrate-binding CitAP domain fused to lipase A of Bacillus subtilis BsLA experimental SAS data
GROMACS model
Sample: citrate-binding CitAP domain fused to lipase A of Bacillus subtilis BsLA dimer, 77 kDa protein
Buffer: 10 mM glycine buffer, 10 mM NaCl, 1 mM sodium citrate, pH: 10
Experiment: SAXS data collected at BM29, ESRF on 2014 Feb 6
A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase. Sci Rep 7:42592 (2017)
Kaschner M, Schillinger O, Fettweiss T, Nutschel C, Krause F, Fulton A, Strodel B, Stadler A, Jaeger KE, Krauss U
RgGuinier 3.3 nm
Dmax 11.7 nm

SASDDJ7 – ...histidine kinase, CitA, fused to lipase EstA

citrate-binding CitAP domain fused to lipase A of Bacillus subtilis BsLA experimental SAS data
DAMMIF model
Sample: citrate-binding CitAP domain fused to lipase A of Bacillus subtilis BsLA dimer, 77 kDa protein
Buffer: 10 mM glycine buffer, 10 mM NaCl, pH: 10
Experiment: SAXS data collected at BM29, ESRF on 2014 Feb 6
A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase. Sci Rep 7:42592 (2017)
Kaschner M, Schillinger O, Fettweiss T, Nutschel C, Krause F, Fulton A, Strodel B, Stadler A, Jaeger KE, Krauss U
RgGuinier 3.3 nm
Dmax 11.8 nm

SASDK48 – Histidine kinase AdeS - DHp-CA Domain

Histidine kinase experimental SAS data
MULTIFOXS model
Sample: Histidine kinase dimer, 51 kDa Acinetobacter baumannii protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Apr 21
Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS. iScience 24(5):102476 (2021)
Ouyang Z, Zheng F, Zhu L, Felix J, Wu D, Wu K, Gutsche I, Wu Y, Hwang PM, She J, Wen Y
RgGuinier 2.8 nm
Dmax 9.1 nm
VolumePorod 85 nm3

SASDK58 – Histidine kinase AdeS - cytoplasmic domain

Histidine kinase experimental SAS data
OTHER model
Sample: Histidine kinase hexamer, 186 kDa Acinetobacter baumannii protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 19
Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS. iScience 24(5):102476 (2021)
Ouyang Z, Zheng F, Zhu L, Felix J, Wu D, Wu K, Gutsche I, Wu Y, Hwang PM, She J, Wen Y
RgGuinier 4.6 nm
Dmax 16.3 nm
VolumePorod 378 nm3

SASDCM9 – Ammonium transporter Ks-Amt5

ammonium sensor/transducer experimental SAS data
CORAL model
Sample: ammonium sensor/transducer trimer, 227 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 1
...histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 511 nm3

SASDCN9 – Ks-Amt5-HK (apo)

Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: ...histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
...histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 71 nm3

SASDCP9 – Ks-Amt5-HK (+10uM APPCP)

Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: ...histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
...histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 53 nm3

SASDCQ9 – Ks-Amt5-HK (+10uM ATP)

Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: ...histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
...histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.2 nm
Dmax 7.7 nm
VolumePorod 53 nm3

SASDCS9 – Ammonium transporter Ks-Amt5 (Batch)

ammonium sensor/transducer experimental SAS data
ammonium sensor/transducer Kratky plot
Sample: ammonium sensor/transducer trimer, 227 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
...histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 4.9 nm
Dmax 17.0 nm
VolumePorod 482 nm3