Human myelin protein P2: from crystallography to time-lapse membrane imaging and neuropathy-associated variants.

Uusitalo M, Klenow MB, Laulumaa S, Blakeley MP, Simonsen AC, Ruskamo S, Kursula P, FEBS J (2021) Europe PMC

SASDLA5 – Wild-type human myelin protein P2

Myelin P2 protein
MWexperimental 12 kDa
MWexpected 15 kDa
VPorod 17 nm3
log I(s) 3.62×10-2 3.62×10-3 3.62×10-4 3.62×10-5
Myelin P2 protein small angle scattering data  s, nm-1
ln I(s)
Myelin P2 protein Guinier plot ln 3.62×10-2 Rg: 1.5 nm 0 (1.5 nm)-2 s2
(sRg)2I(s)/I(0)
Myelin P2 protein Kratky plot 1.104 0 3 sRg
p(r)
Myelin P2 protein pair distance distribution function Rg: 1.5 nm 0 Dmax: 4.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Myelin P2 protein PDB model

log I(s)
 s, nm-1
Myelin P2 protein DAMMIN model

Synchrotron SAXS data from solutions of Wild-type human myelin protein P2 in 20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a wavelength of λ = 0.095 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 9.9 mg/ml was injected at a 0.075 ml/min flow rate onto a GE Superdex 200 Increase 3.2/300 column at 20°C. 21 successive 3 second frames were collected through the SEC peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Myelin P2 protein
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   15.0 kDa
 
UniProt   P02689 (1-132)
Sequence   FASTA
 
PDB code   2WUT