Human myelin protein P2: from crystallography to time-lapse membrane imaging and neuropathy-associated variants.

Uusitalo M, Klenow MB, Laulumaa S, Blakeley MP, Simonsen AC, Ruskamo S, Kursula P
FEBS J (2021 Jun 17)
PMID: 34138518
doi: 10.1111/febs.16079
Submitted to SASBDB: 2021 Apr 15
Published in SASBDB:

SASDLA5 – Wild-type human myelin protein P2

Myelin P2 protein experimental SAS data
PDB model
Sample: Myelin P2 protein monomer, 15 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jul 22
RgGuinier 1.5 nm
Dmax 4.2 nm
VolumePorod 17 nm3

SASDLB5 – CMT mutant i50del of human myelin protein P2

Myelin P2 disease mutant I50del experimental SAS data
DAMMIN model
Sample: Myelin P2 disease mutant I50del monomer, 15 kDa protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jul 22
RgGuinier 1.5 nm
Dmax 4.0 nm
VolumePorod 17 nm3

SASDLC5 – CMT mutant M114T of human myelin protein P2

Human P2 M114T mutant experimental SAS data
DAMMIN model
Sample: Human P2 M114T mutant monomer, 15 kDa protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jul 22
RgGuinier 1.5 nm
Dmax 4.0 nm
VolumePorod 16 nm3

SASDLD5 – CMT mutant V115A of human myelin protein P2

Human P2 V115A mutant experimental SAS data
DAMMIN model
Sample: Human P2 V115A mutant monomer, 15 kDa protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jul 22
RgGuinier 1.5 nm
Dmax 4.0 nm
VolumePorod 17 nm3