Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.

Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M, Nat Commun 15(1):5241 (2024) Europe PMC

SASDQ56 – Retinoic acid-induced protein 2 (RAI2: 303-465) M1 mutant L319A, S320A

Retinoic acid-induced protein 2 (303-465: L319A, S320A)
MWI(0) 18 kDa
MWexpected 17 kDa
VPorod 54 nm3
log I(s) 1.29×10-2 1.29×10-3 1.29×10-4 1.29×10-5
Retinoic acid-induced protein 2 (303-465: L319A, S320A) small angle scattering data  s, nm-1
ln I(s)
Retinoic acid-induced protein 2 (303-465: L319A, S320A) Guinier plot ln 1.29×10-2 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Retinoic acid-induced protein 2 (303-465: L319A, S320A) Kratky plot 1.104 0 √3 sRg
p(r)
Retinoic acid-induced protein 2 (303-465: L319A, S320A) pair distance distribution function Rg: 4.3 nm 0 Dmax: 14.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Retinoic acid-induced protein 2 (RAI2: 303-465) M1 mutant L319A, S320A in 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.60 mg/ml was measured at 20°C. 40 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Retinoic acid-induced protein 2 (303-465: L319A, S320A) (RAI2 (303-465) M1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   17.5 kDa
 
UniProt   Q9Y5P3 (303-465)
Sequence   FASTA