Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.

Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
Nat Commun 15(1):5241 (2024 Jun 19)
PMID: 38898011
doi: 10.1038/s41467-024-49488-3 		Submitted to SASBDB: 2022 Sep 23
Published in SASBDB:

SASDQW5 – C-terminal-binding protein 1 (CtBP1)

C-terminal-binding protein 1 experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 4.3 nm
Dmax 14.0 nm
VolumePorod 279 nm3

SASDQX5 – C-terminal binding protein 1 mutant (CtBP1: C134Y, N138R, R141E, L150W)

C-terminal-binding protein 1 (C134Y, N138R, R141E, L150W) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 (C134Y, N138R, R141E, L150W), 48 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 154 nm3

SASDQY5 – C-terminal binding protein 1 mutant (CtBP1: R266A, D290A, E295A, H315A)

C-terminal-binding protein 1 (R266A, D290A, E295A, H315A) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 (R266A, D290A, E295A, H315A), 47 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 161 nm3

SASDQZ5 – Retinoic acid-induced protein 2 (RAI2: 303-362)

Retinoic acid-induced protein 2 (303-362) experimental SAS data
Retinoic acid-induced protein 2 (303-362) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-362) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 12 nm3

SASDQ26 – Retinoic acid-induced protein 2 (RAI2: 303-362) M1 mutant L319A, S320A

Retinoic acid-induced protein 2 (303-362: L319A, S320A) experimental SAS data
Retinoic acid-induced protein 2 (303-362: L319A, S320A) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-362: L319A, S320A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 10 nm3

SASDQ36 – Retinoic acid-induced protein 2 (RAI2: 303-362) M2 mutant L345A, S346A

Retinoic acid-induced protein 2 (303-362: L345A, S346A) experimental SAS data
Retinoic acid-induced protein 2 (303-362: L345A, S346A) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-362: L345A, S346A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 11 nm3

SASDQ46 – Retinoic acid-induced protein 2 (RAI2: 303-465)

Retinoic acid-induced protein 2 (303-465) experimental SAS data
Retinoic acid-induced protein 2 (303-465) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-465) monomer, 18 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 55 nm3

SASDQ56 – Retinoic acid-induced protein 2 (RAI2: 303-465) M1 mutant L319A, S320A

Retinoic acid-induced protein 2 (303-465: L319A, S320A) experimental SAS data
Retinoic acid-induced protein 2 (303-465: L319A, S320A) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-465: L319A, S320A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 54 nm3

SASDQ66 – Retinoic acid-induced protein 2 (RAI2: 303-465) M2 mutant L345A, S346A

Retinoic acid-induced protein 2 (303-465: L345A, S346A) experimental SAS data
Retinoic acid-induced protein 2 (303-465: L345A, S346A) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-465: L345A, S346A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 55 nm3

SASDQ76 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362)

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 7.4 nm
Dmax 25.0 nm
VolumePorod 889 nm3

SASDQ86 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M1 mutant L319A, S320A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: L319A, S320A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: L319A, S320A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 368 nm3

SASDQ96 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M2 mutant L345A, S346A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: L345A, S346A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 5.5 nm
Dmax 16.0 nm
VolumePorod 396 nm3

SASDQA6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M1+M2 mutant L319A, S320A, L345A, S346A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 4.8 nm
Dmax 15.5 nm
VolumePorod 352 nm3

SASDQB6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) deletion mutant ΔE331-A341

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: ΔE331-A341) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: ΔE331-A341) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: ΔE331-A341) monomer, 6 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 7.2 nm
Dmax 28.0 nm
VolumePorod 912 nm3

SASDQC6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465)

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465) monomer, 18 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 7.4 nm
Dmax 28.0 nm
VolumePorod 978 nm3

SASDQD6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M1 mutant L319A, S320A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465: L319A, S320A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-465: L319A, S320A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465: L319A, S320A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 4.8 nm
Dmax 18.5 nm
VolumePorod 316 nm3

SASDQE6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M2 mutant L345A, S346A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465: L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-465: L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465: L345A, S346A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 4.8 nm
Dmax 18.0 nm
VolumePorod 310 nm3

SASDQF6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M1+M2 mutant L319A, S320A, L345A, S346A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
RgGuinier 5.1 nm
Dmax 17.5 nm
VolumePorod 387 nm3