Goradia N,
Werner S,
Mullapudi E,
Greimeier S,
Bergmann L,
Lang A,
Mertens H
Węglarz A,
Sander S,
Chojnowski G,
Wikman H,
Ohlenschläger O,
von Amsberg G,
Pantel K,
Wilmanns M,
Nat Commun
15(1):5241
(2024)
Europe PMC
SASDQF6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M1+M2 mutant L319A, S320A, L345A, S346A
Synchrotron SAXS
data from solutions of
C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M1+M2 mutant L319A, S320A, L345A, S346A
in
50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 4 m and
at a wavelength of λ = 0.155 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 3.20 mg/ml was measured
at 20°C.
40 successive
0.200 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
