Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures

Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M, Molecules 27(21):7628 (2022) DOI

SASDQQ7 – Bicelles formed by DMPC DMPG and cholate

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol
Temperature 20.0
log I(s)
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol small angle scattering data  s, nm-1
s2I(s)
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot 0 s

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Bicelles formed by DMPC DMPG and cholate in Tris buffered saline, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 20°C. 20 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Data was collected on bicelles formed by DMPC/DMPG (80/20 Mol%) and cholate. The curve shown has a lipid-to-detergent ratio of 5 in Mol%, other ratios are available in the full entry.

Tags: softmatter
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine
Mol. type   Lipid
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid
Mol. type   Lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol
Mol. type   Lipid