Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures

Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M
Molecules 27(21):7628 (2022 Nov 07)

doi: 10.3390/molecules27217628 		Submitted to SASBDB: 2022 Nov 4
Published in SASBDB:

SASDQL7 – Bicelles formed by DMPC DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholineDMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 1

SASDQM7 – Bicelles formed by DMPC cholate

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQN7 – Bicelles formed by DMPC CHAPS

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQP7 – Bicelles formed by DMPC DMPG and CHAPS

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonateDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQQ7 – Bicelles formed by DMPC DMPG and cholate

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQR7 – Bicelles formed by DMPC DMPG and DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholineDMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQS7 – Bicelles formed by POPC CHAPS

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonatePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot
Sample: CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQT7 – Bicelles formed by POPC cholate

Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidPOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot
Sample: Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQU7 – Bicelles formed by POPC DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholinePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQV7 – Bicelles formed by POPC POPG and CHAPS

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonatePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot
Sample: CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQW7 – Bicelles formed by POPC POPG and cholate

Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidPOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot
Sample: Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

SASDQX7 – Bicelles formed by POPC POPG and DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholinePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17