Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.

Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M, Nat Commun 15(1):5241 (2024) Europe PMC

SASDQW5 – C-terminal-binding protein 1 (CtBP1)

C-terminal-binding protein 1
MWI(0) 161 kDa
MWexpected 191 kDa
VPorod 279 nm3
log I(s) 1.04×10-1 1.04×10-2 1.04×10-3 1.04×10-4
C-terminal-binding protein 1 small angle scattering data  s, nm-1
ln I(s)
C-terminal-binding protein 1 Guinier plot ln 1.05×10-1 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
C-terminal-binding protein 1 Kratky plot 1.104 0 √3 sRg
p(r)
C-terminal-binding protein 1 pair distance distribution function Rg: 4.3 nm 0 Dmax: 14 nm

Data validation

Fits and models

log I(s)
 s, nm-1
C-terminal-binding protein 1 CORAL model
Synchrotron SAXS data from solutions of C-terminal-binding protein 1 (CtBP1) in 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.40 mg/ml was measured at 20°C. 40 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

C-terminal-binding protein 1 (CtBP1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   47.7 kDa
 
UniProt   Q13363 (1-440)
Sequence   FASTA
 
PDB ID   6CDF