| MWI(0) | 86 | kDa |
| MWexpected | 47 | kDa |
| VPorod | 161 | nm3 |
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log I(s)
5.60×10-2
5.60×10-3
5.60×10-4
5.60×10-5
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s, nm-1
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Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2.
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M, Nat Commun 15(1):5241 (2024) Europe PMCSASDQY5 – C-terminal binding protein 1 mutant (CtBP1: R266A, D290A, E295A, H315A)
C-terminal-binding protein 1 (R266A, D290A, E295A, H315A)|
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Fits and models
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Synchrotron SAXS
data from solutions of
C-terminal binding protein 1 mutant (CtBP1: R266A, D290A, E295A, H315A)
in
50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.2
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.61 mg/ml was measured
at 20°C.
40 successive
0.200 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Tetramerization mutant. |
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