A hidden property of the iron-sulfur protein in the mononuclear iron-bound state: species-dependent structural ordering induced by magnetic fields.

Arai S, Soga S, Hirai M, Kobayashi R, Masai H, Kimura K, Maeda K, Nagashima H, FEBS J (2025) Europe PMC

SASDUF9 – Mononuclear iron bound form of pigeon ISCA1 under 180 mT magnetic field for 60min

Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial
MWexperimental 15 kDa
MWexpected 15 kDa
VPorod 85 nm3
log I(s) 7.42×10-2 7.42×10-3 7.42×10-4 7.42×10-5
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial Guinier plot ln 7.42×10-2 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot 1.104 0 √3 sRg
p(r)
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Mononuclear iron bound form of pigeon ISCA1 under 180 mT magnetic field for 60min in 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring (Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.155 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.00 mg/ml was measured at 20°C. One 60 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial (clISCA1)
Mol. type   Protein
Organism   Columba livia
Olig. state   Unknown
Mon. MW   14.7 kDa
 
UniProt   P0DN75 (2-132)
Sequence   FASTA