A hidden property of the iron-sulfur protein in the mononuclear iron-bound state: species-dependent structural ordering induced by magnetic fields.

Arai S, Soga S, Hirai M, Kobayashi R, Masai H, Kimura K, Maeda K, Nagashima H, FEBS J (2025) Europe PMC

SASDUH9 – Mononuclear iron bound form of human ISCA1 under 180 mT magnetic field for 1 min

Human Iron-sulfur cluster assembly 1 homolog, mitochondrial
MWexperimental 14 kDa
MWexpected 14 kDa
VPorod 55 nm3
log I(s) 1.56×10-1 1.56×10-2 1.56×10-3 1.56×10-4
Human Iron-sulfur cluster assembly 1 homolog, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Human Iron-sulfur cluster assembly 1 homolog, mitochondrial Guinier plot ln 1.57×10-1 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Human Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot 1.104 0 √3 sRg
p(r)
Human Iron-sulfur cluster assembly 1 homolog, mitochondrial pair distance distribution function Rg: 2.9 nm 0 Dmax: 8.8 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Mononuclear iron bound form of human ISCA1 under 180 mT magnetic field for 1 min in 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring (Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.00 mg/ml was measured at 20°C. One 60 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Human Iron-sulfur cluster assembly 1 homolog, mitochondrial (hsISCA1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Unknown
Mon. MW   14.4 kDa
 
UniProt   Q9BUE6 (2-129)
Sequence   FASTA