A hidden property of the iron-sulfur protein in the mononuclear iron-bound state: species-dependent structural ordering induced by magnetic fields.

Arai S, Soga S, Hirai M, Kobayashi R, Masai H, Kimura K, Maeda K, Nagashima H, FEBS J (2025) Europe PMC

SASDUK9 – Iron-free pigeon ISCA1 under 180mT magnetic field for 60 min

Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial
MWexperimental 15 kDa
MWexpected 15 kDa
log I(s) 5.86×10-2 5.86×10-3 5.86×10-4 5.86×10-5
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial Guinier plot ln 5.87×10-2 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Iron-free pigeon ISCA1 under 180mT magnetic field for 60 min in 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring (Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.155 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.00 mg/ml was measured at 20°C. One 60 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial (clISCA1)
Mol. type   Protein
Organism   Columba livia
Olig. state   Unknown
Mon. MW   14.7 kDa
 
UniProt   P0DN75 (2-132)
Sequence   FASTA