A hidden property of the iron-sulfur protein in the mononuclear iron-bound state: species-dependent structural ordering induced by magnetic fields.

Arai S, Soga S, Hirai M, Kobayashi R, Masai H, Kimura K, Maeda K, Nagashima H, FEBS J (2025) Europe PMC

SASDVB3 – Mononuclear iron bound form of pigeon ISCA1 under geomagnetic condition

Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial
MWexperimental 15 kDa
MWexpected 15 kDa
VPorod 39 nm3
log I(s) 4.38×10-2 4.38×10-3 4.38×10-4 4.38×10-5
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial Guinier plot ln 4.38×10-2 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot 1.104 0 √3 sRg
p(r)
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial pair distance distribution function Rg: 2.5 nm 0 Dmax: 7.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial DAMMIN model
Synchrotron SAXS data from solutions of Mononuclear iron bound form of pigeon ISCA1 under geomagnetic condition in 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring (Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 3.0 m and at a wavelength of λ = 0.155 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.00 mg/ml was measured at 20°C. One 60 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial (clISCA1)
Mol. type   Protein
Organism   Columba livia
Olig. state   Unknown
Mon. MW   14.7 kDa
 
UniProt   P0DN75 (2-132)
Sequence   FASTA