Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.

Tripepi A, Shakoor H, Zlobina M, Klumpler T Kubíčková M, Houser J, Klapetek P, Lukavsky PJ, Protein Sci 35(7):e70669 (2026) Europe PMC

SASDWH9 – Double-stranded RNA-binding protein Staufen homolog 1

Double-stranded RNA-binding protein Staufen homolog 1 (82-577)
MWexperimental 69 kDa
MWexpected 55 kDa
VPorod 92 nm3
log I(s) 4.87×102 4.87×101 4.87×100 4.87×10-1
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) small angle scattering data  s, nm-1
ln I(s)
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) Guinier plot ln 4.88×102 Rg: 5 nm 0 (5 nm)-2 s2
(sRg)2I(s)/I(0)
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) Kratky plot 1.104 0 √3 sRg
p(r)
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) pair distance distribution function Rg: 4.9 nm 0 Dmax: 15.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Double-stranded RNA-binding protein Staufen homolog 1 Rg histogram Rg, nm
Double-stranded RNA-binding protein Staufen homolog 1 Rg histogram Rg, nm
Double-stranded RNA-binding protein Staufen homolog 1 Rg histogram Rg, nm
Double-stranded RNA-binding protein Staufen homolog 1 Rg histogram Rg, nm
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) OTHER [STATIC IMAGE] model
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (82-577) EOM/RANCH model
Synchrotron SAXS data from solutions of Double-stranded RNA-binding protein Staufen homolog 1 in 50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanol, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123983 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 5 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 104 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Double-stranded RNA-binding protein Staufen homolog 1 (82-577) (hStau1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Other
Mon. MW   55.2 kDa
 
UniProt   O95793 (82-577)
Sequence   FASTA