Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.

Tripepi A, Shakoor H, Zlobina M, Klumpler T, Kubíčková M, Houser J, Klapetek P, Lukavsky PJ
Protein Sci 35(7):e70669 (2026 Jul)
PMID: 42300088
doi: 10.1002/pro.70669 		Submitted to SASBDB: 2025 Feb 28
Published in SASBDB:

SASDWK9 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif

Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) experimental SAS data
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif Rg histogram
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif Rg histogram
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif Rg histogram
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif Rg histogram
Sample: Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488), 52 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanol, pH: 7
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2020 Apr 5
RgGuinier 5.1 nm
Dmax 13.8 nm
VolumePorod 105 nm3

SASDWM9 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2

Double-stranded RNA-binding protein Staufen homolog 1 (178-577) experimental SAS data
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 Rg histogram
Sample: Double-stranded RNA-binding protein Staufen homolog 1 (178-577), 44 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 23
RgGuinier 4.6 nm
Dmax 13.7 nm
VolumePorod 89 nm3

SASDWN9 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated Staufen-swapping (ΔSSM) motif

Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488) experimental SAS data
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated Staufen-swapping (ΔSSM) motif Rg histogram
Sample: Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488) monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 17
RgGuinier 4.6 nm
Dmax 16.9 nm
VolumePorod 99 nm3

SASDWJ9 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated RNA-binding domain 3

Double-stranded RNA-binding protein Staufen homolog 1 (286-577) experimental SAS data
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated RNA-binding domain 3 Rg histogram
Sample: Double-stranded RNA-binding protein Staufen homolog 1 (286-577), 32 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 23
RgGuinier 4.0 nm
Dmax 12.9 nm
VolumePorod 46 nm3

SASDWL9 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated RNA-binding domain 3 and truncated Staufen-swapping (ΔSSM) motif

Double-stranded RNA-binding protein Staufen homolog 1 (286-577 + Δ454-488) experimental SAS data
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated RNA-binding domain 3 and truncated Staufen-swapping (ΔSSM) motif Rg histogram
Sample: Double-stranded RNA-binding protein Staufen homolog 1 (286-577 + Δ454-488) monomer, 29 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 17
RgGuinier 4.1 nm
Dmax 19.5 nm
VolumePorod 64 nm3

SASDWH9 – Double-stranded RNA-binding protein Staufen homolog 1

Double-stranded RNA-binding protein Staufen homolog 1 (82-577) experimental SAS data
Double-stranded RNA-binding protein Staufen homolog 1 Rg histogram
Double-stranded RNA-binding protein Staufen homolog 1 Rg histogram
Double-stranded RNA-binding protein Staufen homolog 1 Rg histogram
Double-stranded RNA-binding protein Staufen homolog 1 Rg histogram
Sample: Double-stranded RNA-binding protein Staufen homolog 1 (82-577), 55 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 23
RgGuinier 5.0 nm
Dmax 15.1 nm
VolumePorod 92 nm3

SASDUS5 – Human Double-stranded RNA-binding protein Staufen homolog 1

Double-stranded RNA-binding protein Staufen homolog 1 (Δ1-81) experimental SAS data
Human Double-stranded RNA-binding protein Staufen homolog 1 Rg histogram
Sample: Double-stranded RNA-binding protein Staufen homolog 1 (Δ1-81) dimer, 110 kDa Homo sapiens protein
Buffer: 50 mM TRIS, 300 mM NaCl, 3.8 mM β-mercaptoethanol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 23
RgGuinier 5.0 nm
Dmax 14.1 nm
VolumePorod 86 nm3