SASDWL9 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated RNA-binding domain 3 and truncated Staufen-swapping (ΔSSM) motif

MWexperimental 36 kDa MWexpected 29 kDa VPorod 64 nm3 log I(s) 2.60×103 2.60×102 2.60×101 2.60×100  s, nm-1 Log plot Log-Log plot

ln I(s) ln 2.60×103 Rg: 4.1 nm 0 (4.1 nm)-2 s2 (sRg)2I(s)/I(0) 1.104 0 √3 sRg p(r) Rg: 4.5 nm 0 Dmax: 19.5 nm

Fits and models

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log I(s)  s, nm-1 +3 Δ/σ -3  s, nm-1 Fit validation Metric Value pcormap χ² 0.487 0.990 Worse Better

Rg, nm

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Synchrotron SAXS data from solutions of Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated RNA-binding domain 3 and truncated Staufen-swapping (ΔSSM) motif in 50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124026 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 95.00 μl sample at 7.4 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 63 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Double-stranded RNA-binding protein Staufen homolog 1 (286-577 + Δ454-488) (dsRBD4-EndΔSSM) Mol. type   Protein Organism   Homo sapiens Olig. state   Monomer Mon. MW   28.7 kDa   UniProt   O95793 (286-577) Sequence   FASTA