Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.

Tripepi A, Shakoor H, Zlobina M, Klumpler T Kubíčková M, Houser J, Klapetek P, Lukavsky PJ, Protein Sci 35(7):e70669 (2026) Europe PMC

SASDWN9 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated Staufen-swapping (ΔSSM) motif

Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488)
MWexperimental 60 kDa
MWexpected 41 kDa
VPorod 99 nm3
log I(s) 5.42×103 5.42×102 5.42×101 5.42×100
Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488) small angle scattering data  s, nm-1
ln I(s)
Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488) Guinier plot ln 5.43×103 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488) Kratky plot 1.104 0 √3 sRg
p(r)
Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488) pair distance distribution function Rg: 4.9 nm 0 Dmax: 16.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated Staufen-swapping (ΔSSM) motif Rg histogram Rg, nm
Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488) EOM/RANCH model
Synchrotron SAXS data from solutions of Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 and truncated Staufen-swapping (ΔSSM) motif in 50 mM Tris-HCl, 150 mM NaCl, 3.6 mM β-mercaptoethanol, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123982 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 3.7 mg/ml was injected at a 0.40 ml/min flow rate onto a GE Superdex 75 5/150 column at 20°C. 38 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Double-stranded RNA-binding protein Staufen homolog 1 (178-577 + Δ454-488) (dsRBD3-End∆SSM)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   40.7 kDa
 
UniProt   O95793 (178-577)
Sequence   FASTA