Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling.

Fisher H, Sutton EJ, Oldham RJ, Bradshaw RT, Duriez PJ, Frendéus B, Larsson G, Manfredi G, Martin-Fernandez ML, Mockridge I, Needham SR, Rolfe DJ, Orr CM, Patel K, Roghanian A, Simpson A, Tully MD, Teige I, Tornberg UC, Tynan CJ, Pendower A, Kim J, Tennenhouse A, Fleishman SJ, Essex JW, Tews I, Cragg MS
Immunity (2026 Jun 25)

PMID: 42349409 doi: 10.1016/j.immuni.2026.05.019

Submitted to SASBDB: 2024 Apr 15 Published in SASBDB: 2026 Apr 15

SASDU77 – Human IgG1 F(ab') 5C05 Sample: Human IgG1 F(ab') 5C05 monomer, 46 kDa Homo sapiens protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 May 9 RgGuinier 2.5 nm Dmax 7.7 nm VolumePorod 59 nm3

SASDU87 – Deglycosylated Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 5C05 F(ab') Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein Human IgG1 F(ab') 5C05 monomer, 46 kDa Homo sapiens protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 May 18 RgGuinier 3.5 nm Dmax 14.0 nm VolumePorod 95 nm3

SASDU97 – Human IgG1 F(ab') 6C11 Sample: Human IgG1 F(ab') 6C11 monomer, 24 kDa Homo sapiens protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 May 9 RgGuinier 2.5 nm Dmax 7.6 nm VolumePorod 56 nm3

SASDUA7 – Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 6C11 F(ab') Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein Human IgG1 F(ab') 6C11 monomer, 24 kDa Homo sapiens protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 May 9 RgGuinier 3.4 nm Dmax 12.0 nm VolumePorod 110 nm3

SASDUB7 – Human IgG1 F(ab') 6G11 Sample: Human IgG1 F(ab') 6G11 monomer, 25 kDa Homo sapiens protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 5 RgGuinier 2.6 nm Dmax 8.5 nm VolumePorod 60 nm3

SASDUC7 – Deglycosylated Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 6G11 F(ab') Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein Human IgG1 F(ab') 6G11 monomer, 25 kDa Homo sapiens protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 9 RgGuinier 3.5 nm Dmax 13.5 nm VolumePorod 97 nm3

SASDUD7 – Human IgG1 F(ab') 7C07 Sample: Human IgG1 F(ab') 7C07 monomer, 25 kDa Homo sapiens protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 5 RgGuinier 2.6 nm Dmax 8.2 nm VolumePorod 59 nm3

SASDUE7 – Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 7C07 F(ab') Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein Human IgG1 F(ab') 7C07 monomer, 25 kDa Homo sapiens protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 5 RgGuinier 3.6 nm Dmax 13.5 nm VolumePorod 110 nm3

SASDUF7 – Deglycosylated Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 6G08 F(ab') Sample: Low affinity immunoglobulin gamma Fc region receptor II-b monomer, 20 kDa Homo sapiens protein Human IgG1 F(ab') 6G08 monomer, 24 kDa Homo sapiens protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 14 RgGuinier 3.3 nm Dmax 12.0 nm VolumePorod 96 nm3