Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation.

Lockhart-Cairns MP, Cain SA, Dajani R, Steer R, Thomson J, Alanazi YF, Kielty CM, Baldock C
Matrix Biol (2022 Feb 2)

PMID: 35122964 doi: 10.1016/j.matbio.2022.01.005

Submitted to SASBDB: 2021 Dec 23 Published in SASBDB: 2022 Feb 16

SASDNR3 – Fibrillin-1 fragment PF3 Sample: Fibrillin-1 PF3 monomer, 78 kDa Homo sapiens protein Buffer: Tris buffered saline, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 12 RgGuinier 5.4 nm Dmax 24.0 nm VolumePorod 141 nm3

SASDNS3 – Latent-transforming growth factor beta-binding protein 1 C-terminus Sample: Latent-transforming growth factor beta-binding protein 1 monomer, 44 kDa Homo sapiens protein Buffer: Tris buffered saline, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 12 RgGuinier 4.7 nm Dmax 20.5 nm VolumePorod 102 nm3

SASDNT3 – Fibrillin-1 fragment PF3 crosslinked to latent transforming growth factor-beta-binding protein 1 C-terminus by transglutaminase 2 Sample: Latent-transforming growth factor beta-binding protein 1 monomer, 44 kDa Homo sapiens protein Fibrillin-1 PF3 monomer, 78 kDa Homo sapiens protein Buffer: 10 mM Hepes, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 20 RgGuinier 7.4 nm Dmax 29.6 nm VolumePorod 759 nm3