Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis.

Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L
BMC Biol 20(1):147 (2022 Jun 21)

PMID: 35729566 doi: 10.1186/s12915-022-01337-9

Submitted to SASBDB: 2022 May 4 Published in SASBDB: 2022 May 24

SASDNK9 – Monomer of the full-length apo-polyketide synthase Pks13 of Mycobacterium tuberculosis Sample: Polyketide synthase Pks13 monomer, 188 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2013 Mar 1 RgGuinier 7.3 nm Dmax 24.8 nm VolumePorod 480 nm3

SASDNL9 – Full length apo-polyketide synthase Pks13(S1533A) with Ser801 loaded by a C16-carbon chain Sample: Polyketide synthase Pks13 dimer, 376 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2011 Nov 23 RgGuinier 10.3 nm Dmax 36.0 nm VolumePorod 2300 nm3

SASDNM9 – Thioesterase domain of polyketide synthase Pks13 at neutral pH Sample: Polyketide synthase Pks13 monomer, 33 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1 RgGuinier 1.9 nm Dmax 5.5 nm VolumePorod 56 nm3

SASDNN9 – di-domain sub fragment ACP2TE of polyketide synthase Pks13 at neutral pH Sample: Polyketide synthase Pks13 monomer, 62 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris-HCl 50 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1 RgGuinier 3.2 nm Dmax 12.0 nm VolumePorod 80 nm3

SASDNP9 – AT52: acyltransferase domain and insertion domain of polyketide synthase Pks13 at neutral pH Sample: Polyketide synthase Pks13 monomer, 53 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris-HCl, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1 RgGuinier 3.5 nm Dmax 12.0 nm VolumePorod 115 nm3

SASDNQ9 – Acyltransferase domain of polyketide synthase Pks13 at neutral pH Sample: Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris-HCl, 300 mM NaCl, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2011 Jun 3 RgGuinier 2.8 nm Dmax 8.0 nm VolumePorod 85 nm3

SASDNR9 – Subfragment ACP1KSAT tridomain of polyketide synthase Pks13 at neutral pH Sample: Polyketide synthase Pks13 monomer, 116 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris-HCl 50 mM NaCl, pH: 8.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1 RgGuinier 3.8 nm Dmax 12.9 nm VolumePorod 186 nm3

SASDNS9 – KS domain of polyketide synthase Pks13 at neutral pH Sample: Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2012 Dec 7 RgGuinier 2.9 nm Dmax 10.0 nm VolumePorod 82 nm3

SASDNT9 – Subfragment KSAT of Polyketide synthase Pks13 at neutral pH Sample: Polyketide synthase Pks13 monomer, 104 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2008 Sep 20 RgGuinier 3.8 nm Dmax 13.0 nm VolumePorod 155 nm3

SASDNU9 – Mycocerosic acid synthase in its apo form and at neutral pH Sample: Mycocerosic acid synthase monomer, 224 kDa Mycobacterium bovis (strain … protein Buffer: 50 mM Tris-HCl, 50 mM NaCl, 10% glycerol, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2013 Oct 1 RgGuinier 5.7 nm Dmax 21.0 nm VolumePorod 420 nm3

SASDNV9 – Phenolpthiocerol synthase A of BCG at neutral pH Sample: Phenolpthiocerol synthesis type-I polyketide synthase ppsA monomer, 199 kDa Mycobacterium bovis (strain … protein Buffer: 50 mM Tris-HCl, 500 mM NaCl, 2 mM EDTA, 10% glycerol, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2012 Jun 14 RgGuinier 6.7 nm Dmax 20.2 nm VolumePorod 680 nm3