DPY30 acts as an ASH2L-specific stabilizer to stimulate the enzyme activity of MLL family methyltransferases on different substrates.

Zhao L, Huang N, Mencius J, Li Y, Xu Y, Zheng Y, He W, Li N, Zheng J, Zhuang M, Quan S, Chen Y
iScience 25(9):104948 (2022 Sep 16)

PMID: 36065180 doi: 10.1016/j.isci.2022.104948

Submitted to SASBDB: 2022 May 26 Published in SASBDB: 2022 Aug 8

SASDPT3 – Histone-lysine N-methyltransferase multi protein complex: MLL1-WDR5-RBBP5-ASH2L Sample: Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein WD repeat-containing protein 5 monomer, 37 kDa Homo sapiens protein Retinoblastoma-binding protein 5 monomer, 59 kDa Homo sapiens protein Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TECP, pH: 7.4 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1 RgGuinier 5.2 nm Dmax 18.5 nm VolumePorod 386 nm3

SASDPU3 – Histone-lysine N-methyltransferase multi protein complex bound to protein dpy-30 homologue: MLL1-WDR5-RBBP5-ASH2L-DPY30 Sample: Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein WD repeat-containing protein 5 monomer, 37 kDa Homo sapiens protein Retinoblastoma-binding protein 5 monomer, 59 kDa Homo sapiens protein Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein Protein dpy-30 homolog dimer, 23 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TECP, pH: 7.4 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1 RgGuinier 5.4 nm Dmax 17.5 nm VolumePorod 411 nm3