The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics.

Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
Biochemistry (2022 May 10)
PMID: 35535990
doi: 10.1021/acs.biochem.2c00072 		Submitted to SASBDB: 2021 Jul 11
Published in SASBDB:

SASDLM8 – Human apo Nocturnin - Extended

Nocturnin experimental SAS data
Human apo Nocturnin - Extended Rg histogram
Sample: Nocturnin monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
RgGuinier 2.9 nm
Dmax 12.3 nm
VolumePorod 85 nm3

SASDLN8 – Human apo Nocturnin - Compact

Nocturnin experimental SAS data
Human apo Nocturnin - Compact Rg histogram
Sample: Nocturnin monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
RgGuinier 2.4 nm
Dmax 9.6 nm
VolumePorod 76 nm3

SASDLP8 – Human apo Nocturnin - Deletion construct (Δ107-120) - Extended

Nocturnin - Deletion construct - Δ107-120 experimental SAS data
Human apo Nocturnin - Deletion construct (Δ107-120) - Extended Rg histogram
Sample: Nocturnin - Deletion construct - Δ107-120 monomer, 40 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
RgGuinier 2.8 nm
Dmax 11.2 nm
VolumePorod 72 nm3

SASDLQ8 – Human apo Nocturnin - Deletion construct (Δ107-120) - Compact

Nocturnin - Deletion construct - Δ107-120 experimental SAS data
Human apo Nocturnin - Deletion construct (Δ107-120) - Compact Rg histogram
Sample: Nocturnin - Deletion construct - Δ107-120 monomer, 40 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 80 nm3

SASDLR8 – Human apo Nocturnin - N terminus truncated (118-NOCT)

Nocturnin - N terminus truncated (118-NOCT) experimental SAS data
EOM/RANCH model
Sample: Nocturnin - N terminus truncated (118-NOCT) monomer, 36 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
RgGuinier 2.3 nm
Dmax 10.3 nm
VolumePorod 63 nm3