Structural basis for SHOC2 modulation of RAS signalling.

Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J
Nature (2022 Jun 29)

PMID: 35768504 doi: 10.1038/s41586-022-04838-3

Submitted to SASBDB: 2021 Sep 20 Published in SASBDB: 2022 Apr 24

SASDMB5 – Leucine-rich repeat protein SHOC-2 Sample: Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein Buffer: 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jun 20 RgGuinier 3.2 nm Dmax 11.2 nm VolumePorod 85 nm3

SASDMC5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C) Sample: Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein Serine/threonine-protein phosphatase PP1-gamma catalytic subunit monomer, 37 kDa Homo sapiens protein Buffer: 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 20 RgGuinier 3.3 nm Dmax 11.7 nm VolumePorod 132 nm3

SASDMD5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C) and GTPase KRas Sample: Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein Serine/threonine-protein phosphatase PP1-gamma catalytic subunit monomer, 37 kDa Homo sapiens protein GTPase KRas monomer, 19 kDa Homo sapiens protein Buffer: 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 20 RgGuinier 3.3 nm Dmax 11.7 nm VolumePorod 156 nm3

SASDME5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C) and Ras-related protein M-Ras Sample: Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein Serine/threonine-protein phosphatase PP1-gamma catalytic subunit monomer, 37 kDa Homo sapiens protein Ras-related protein M-Ras monomer, 24 kDa Homo sapiens protein Buffer: 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 20 RgGuinier 3.5 nm Dmax 12.4 nm VolumePorod 215 nm3