Probing DNA transposition complex formation by AFM-based force spectroscopy

Alexander Shkumatov Submitted to SASBDB: 2021 Oct 4
Published in SASBDB:

SASDMR5 – Tn3 family transposase (TnpA WT)

TnpA transposase experimental SAS data
GASBOR model
Sample: TnpA transposase dimer, 234 kDa Bacillus thuringiensis serovar … protein
Buffer: 50 mM HEPES, 200 mM NaCl, 100 mM L-Arg HCL, pH: 7.9
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Nov 2
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 480 nm3