X-ray Characterization of Conformational Changes of Human Apo- and Holo-Transferrin

Campos-Escamilla C, Siliqi D, Gonzalez-Ramirez L, Lopez-Sanchez C, Gavira J, Moreno A
International Journal of Molecular Sciences 22(24):13392 (2021 Dec 13)

doi: 10.3390/ijms222413392 		Submitted to SASBDB: 2021 Oct 25
Published in SASBDB:

SASDMP7 – Apo-serotransferrin at pH 8.0

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (Buffer APO-Tf-1), pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
RgGuinier 3.1 nm
Dmax 9.4 nm
VolumePorod 106 nm3

SASDMN7 – Apo-serotransferrin at pH 7.0

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (APO Buffer), pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
RgGuinier 3.1 nm
Dmax 9.6 nm
VolumePorod 106 nm3

SASDMM7 – Apo-serotransferrin at pH 5.5

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl, (APO Buffer), pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
RgGuinier 3.1 nm
Dmax 9.8 nm
VolumePorod 106 nm3

SASDMS7 – HOLO-serotransferrin at pH 8.0 in the presence of iron

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (Buffer APO-Tf-1), pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
RgGuinier 3.2 nm
Dmax 13.1 nm
VolumePorod 106 nm3

SASDMR7 – HOLO-serotransferrin at pH 7.0 in the presence of iron

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (APO Buffer), pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
RgGuinier 3.2 nm
Dmax 13.5 nm
VolumePorod 103 nm3

SASDMQ7 – HOLO-serotransferrin at pH 5.5 in the presence of iron

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl, (APO Buffer), pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
RgGuinier 3.3 nm
Dmax 14.6 nm
VolumePorod 107 nm3