Small-angle X-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model

Caporaletti F, Pietras Z, Morad V, Mårtensson L, Gabel F, Wallner B, Martel A, Sunnerhagen M
Biophysical Journal (2022 Dec)

doi: 10.1016/j.bpj.2022.11.2949

Submitted to SASBDB: 2021 Dec 1 Published in SASBDB: 2022 Dec 6

SASDMG9 – Multidrug resistance operon repressor (MexR) of the MexAB-OprM multidrug efflux pump operon of Pseudomonas aeruginosa Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein Buffer: 20mM HEPES, 150mM NaCl, 10mM DTT, 1% v/v glycerol, pH: 7.1 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Nov 23 RgGuinier 2.3 nm Dmax 7.7 nm VolumePorod 56 nm3

SASDMH9 – Pseudomonas aeruginosa Multidrug resistance operon repressor (MexR) in complex with 34bp dsDNA binding sequence (SANS data at 0% D2O) Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein 34 base pair double-stranded DNA monomer, 21 kDa synthetic construct DNA Buffer: 20mM NaPO4, 150 mM NaCl, 10 mM DTT, pH: 7.1 Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 May 30 RgGuinier 2.9 nm Dmax 7.8 nm VolumePorod 79 nm3