Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity

Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R
Journal of Virology 86(8):4444-4454 (2012 Feb 08)

doi: 10.1128/JVI.06635-11 		Submitted to SASBDB: 2021 Dec 6
Published in SASBDB:

SASDMJ9 – Plasmid pET11d- 229E710 encoding C-terminally His6-tagged HCoV-229E Nsp7-10 polyprotein

Replicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Buffer: 10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2
RgGuinier 2.1 nm

SASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)

Replicase polyprotein 1aReplicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Replicase polyprotein 1a monomer, 22 kDa Severe acute respiratory … protein
Buffer: 10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2
RgGuinier 3.0 nm