Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179

Patil D, Pantalone S, Cao Y, Laboute T, Novick S, Singh S, Savino S, Faravelli S, Magnani F, Griffin P, Singh A, Forneris F, Martemyanov K
Science Signaling 16(795) (2023 Jul 25)

doi: 10.1126/scisignal.add9539 		Submitted to SASBDB: 2022 Mar 26
Published in SASBDB:

SASDN78 – Pikachurin N-terminal FnIII(1-2) fragment (SEC-SAXS)

Pikachurin N-terminal FnIII(1-2) domains experimental SAS data
GASBOR model
Sample: Pikachurin N-terminal FnIII(1-2) domains monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
RgGuinier 3.4 nm
Dmax 16.4 nm
VolumePorod 44 nm3

SASDN68 – Pikachurin C-terminal LG3 domain (SEC-SAXS)

Pikachurin experimental SAS data
GASBOR model
Sample: Pikachurin monomer, 22 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
RgGuinier 2.2 nm
Dmax 8.2 nm
VolumePorod 48 nm3

SASDN88 – Pikachurin C-terminal EGF-LG3 fragment (SEC-SAXS)

Pikachurin experimental SAS data
GASBOR model
Sample: Pikachurin monomer, 26 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 52 nm3