SAXS/WAXS data of conformationally flexible ribose binding protein

Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B
Data in Brief :109932 (2023 Dec)

doi: 10.1016/j.dib.2023.109932
Submitted to SASBDB: 2023 Sep 5
Published in SASBDB:

SASDTB2 – WAXS data of poly-histidine tagged Ribose binding protein (16 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
RgGuinier 2.5 nm
Dmax 12.0 nm
VolumePorod 36 nm3

SASDTC2 – WAXS data of poly-histidine tagged Ribose binding protein (20 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
RgGuinier 2.2 nm
Dmax 12.0 nm
VolumePorod 36 nm3

SASDTD2 – WAXS data of poly-histidine tagged Ribose binding protein (17 mg/mL) from Escherichia coli in the presence of 5 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: Tris 50 mM, 50 mM NaCl, 10% glycerol, 5 mM ribose, pH: 7
Experiment: SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 9
RgGuinier 1.9 nm
Dmax 10.7 nm
VolumePorod 25 nm3

SASDSB9 – SAXS data of poly-histidine tagged Ribose Binding Protein (9 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
RgGuinier 2.1 nm
Dmax 8.6 nm
VolumePorod 35 nm3

SASDSC9 – SAXS data of poly-histidine tagged Ribose Binding Protein (6 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
RgGuinier 2.1 nm
Dmax 8.4 nm
VolumePorod 37 nm3

SASDSD9 – SAXS data of poly-histidine tagged Ribose Binding Protein (3 mg/mL) from Escherichia coli

Ribose import binding protein RbsB experimental SAS data
Ribose import binding protein RbsB Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 39 nm3

SASDSE9 – SAXS data of poly-histidine tagged Ribose Binding Protein (9 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
RgGuinier 2.0 nm
Dmax 8.3 nm
VolumePorod 35 nm3

SASDSF9 – SAXS data of poly-histidine tagged Ribose Binding Protein (6 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
RgGuinier 2.0 nm
Dmax 7.6 nm
VolumePorod 36 nm3

SASDSG9 – SAXS data of poly-histidine tagged Ribose Binding Protein (3 mg/mL) from Escherichia coli in the presence of 1 mM ribose

Ribose import binding protein RbsBRibose experimental SAS data
Ribose import binding protein RbsB Ribose Kratky plot
Sample: Ribose import binding protein RbsB monomer, 30 kDa Escherichia coli (strain … protein
Ribose monomer, 0 kDa
Buffer: 50 mM Tris, 50 mM NaCl, 10% glycerol, 1 mM ribose, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 39 nm3