A SEC-SAXS analysis of SARS-CoV-2 nsp14 apo or in complex with vhh and/or nsp10

Pierre Gauffre

Submitted to SASBDB: 2024 Oct 23 Published in SASBDB: 2026 Oct 23

SASDVU8 – nsp14 from SARS-CoV-2 Sample: Replicase polyprotein 1ab (Guanine-N7 methyltransferase nsp14) monomer, 60 kDa Severe acute respiratory … protein Buffer: 25 mM HEPES, 300 mM NaCl, 5 mM CaCl2, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Feb 17 RgGuinier 3.1 nm Dmax 11.5 nm VolumePorod 85 nm3

SASDVV8 – nsp14 and nsp10 from SARS-CoV-2 Sample: Replicase polyprotein 1ab (Guanine-N7 methyltransferase nsp14) monomer, 60 kDa Severe acute respiratory … protein Replicase polyprotein 1ab (Non-structural protein 10) monomer, 17 kDa Severe acute respiratory … protein Buffer: 25 mM HEPES, 300 mM NaCl, 5 mM CaCl2, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Feb 17 RgGuinier 3.2 nm Dmax 12.3 nm VolumePorod 85 nm3

SASDVW8 – nsp14 and nsp10 from SARS-CoV-2 with vhh Sample: Replicase polyprotein 1ab (Guanine-N7 methyltransferase nsp14) monomer, 60 kDa Severe acute respiratory … protein Replicase polyprotein 1ab (Non-structural protein 10) monomer, 17 kDa Severe acute respiratory … protein Vhh anti-nsp14 SARS-CoV-2 monomer, 14 kDa protein Buffer: 25 mM HEPES, 300 mM NaCl, 5 mM CaCl2, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 17 RgGuinier 3.8 nm Dmax 19.7 nm VolumePorod 147 nm3

SASDVX8 – nsp14 from SARS-CoV-2 with vhh Sample: Replicase polyprotein 1ab (Guanine-N7 methyltransferase nsp14) monomer, 60 kDa Severe acute respiratory … protein Vhh anti-nsp14 SARS-CoV-2 monomer, 14 kDa protein Buffer: 25 mM HEPES, 300 mM NaCl, 5 mM CaCl2, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 17 RgGuinier 3.4 nm Dmax 12.3 nm VolumePorod 110 nm3