Functional Characterization of Arabidopsis α-Amylase3 (AMY3): Amylose Specificity and Structural Insights into Its Duplex Carbohydrate-Binding Module

Christopher Berndsen

Submitted to SASBDB: 2025 Sep 12 Published in SASBDB: 2026 Sep 12

SASDX79 – carbohydrate binding duplex of AMY3 (alpha-amylase 3) Sample: Carbohydrate binding modules of AMY3 (alpha-amylase 3, chloroplastic) dimer, 77 kDa Arabidopsis thaliana protein Buffer: 100 mM potassium phosphate, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2024 Jun 19 RgGuinier 3.2 nm Dmax 9.9 nm VolumePorod 110 nm3

SASDX99 – cross-linked carbohydrate binding duplex of AMY3 (alpha-amylase 3) Sample: Cross-linked carbohydrate binding modules of AMY3 (alpha-amylase 3, chloroplastic) dimer, 78 kDa Arabidopsis thaliana protein Buffer: 100 mM potassium phosphate, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2024 Jun 19 RgGuinier 3.3 nm Dmax 10.6 nm VolumePorod 106 nm3

SASDX89 – AMY3 (alpha-amylase 3) catalytic domain Sample: Amylase domain of AMY3 (alpha-amylase 3, chloroplastic) monomer, 47 kDa Arabidopsis thaliana protein Buffer: 100 mM potassium phosphate, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2025 Jun 4 RgGuinier 2.4 nm Dmax 8.2 nm VolumePorod 57 nm3