Conformational dynamics of the membrane-anchored foldase LipH from Pseudomonas aeruginosa governs recognition and release of its client lipase

Busch M, Loschwitz J, Papadopoulos A, Reiners J, Steinchen W, Calvagna V, Smits S, Jaeger K, Kedrov A
Journal of Biological Chemistry :113266 (2026 Jun)

doi: 10.1016/j.jbc.2026.113266

Submitted to SASBDB: 2026 Mar 8 Published in SASBDB: 2026 Jun 9

SASDYM9 – Lipase chaperone (LipH) VD Sample: Lipase chaperone monomer, 38 kDa Pseudomonas aeruginosa (strain … protein Buffer: 50 mM Tris, 100 mM NaCl, 100 µM TCEP, 5% glycerol,, pH: 8 Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2019 Nov 18 RgGuinier 3.2 nm Dmax 12.6 nm VolumePorod 70 nm3

SASDYN9 – Lipase chaperone (LipH) VD : Triacylglycerol lipase (LipA) complex Sample: Lipase chaperone monomer, 38 kDa Pseudomonas aeruginosa (strain … protein Triacylglycerol lipase monomer, 30 kDa Pseudomonas aeruginosa (strain … protein Buffer: 5 mM Tris, 5 mM glycine, 1 mM CaCl2, 5% glycerol,, pH: 9 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14 RgGuinier 3.1 nm Dmax 11.8 nm VolumePorod 110 nm3

SASDYP9 – Lipase chaperone (LipH) VD : Triacylglycerol lipase (LipA) delta81 complex Sample: Lipase chaperone monomer, 38 kDa Pseudomonas aeruginosa (strain … protein Triacylglycerol lipase monomer, 21 kDa Pseudomonas aeruginosa (strain … protein Buffer: 5 mM Tris, 5 mM glycine, 1 mM CaCl2, 5% glycerol,, pH: 9 Experiment: SAXS data collected at BM29, ESRF on 2024 Sep 10 RgGuinier 3.5 nm Dmax 11.7 nm VolumePorod 104 nm3