Open or closed? Structural characterization of plasma-derived and recombinant human plasminogen across multiple resolution scales.

Kasiarova L, Verma N, Pinkas M, Henek T, Klumpler T, Chovancikova M, Reutova H, Kasparek P, Hlozankova M, Damborsky J, Novacek J, Hernychova L, Marek M
Int J Biol Macromol :154885 (2026 Oct 19)

PMID: 42896770 doi: 10.1016/j.ijbiomac.2026.154885

Submitted to SASBDB: 2026 Mar 12 Published in SASBDB: 2026 Oct 23

SASDZU3 – Plasminogen Athens Sample: Plasminogen monomer, 88 kDa Homo sapiens protein Buffer: 10mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2025 Mar 14 RgGuinier 5.4 nm Dmax 16.8 nm VolumePorod 119 nm3

SASDZV3 – Plasminogen Sigma Sample: Plasminogen monomer, 88 kDa Homo sapiens protein Buffer: 10mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2025 Jan 14 RgGuinier 3.4 nm Dmax 10.2 nm VolumePorod 132 nm3

SASDZW3 – Plasminogen Innovative Research Sample: Plasminogen monomer, 88 kDa Homo sapiens protein Buffer: 10mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2026 Jan 22 RgGuinier 5.7 nm Dmax 19.3 nm VolumePorod 276 nm3

SASDZX3 – Plasminogen Ivg Sample: Plasminogen monomer, 88 kDa Homo sapiens protein Buffer: 10mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2026 Jan 22 RgGuinier 3.6 nm Dmax 10.5 nm VolumePorod 142 nm3

SASDZY3 – Plasminogen Roche Sample: Plasminogen monomer, 88 kDa Homo sapiens protein Buffer: 10mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2026 Jan 21 RgGuinier 3.0 nm Dmax 9.3 nm VolumePorod 102 nm3

SASDZZ3 – Plasmin Sample: Plasminogen monomer, 88 kDa Homo sapiens protein Buffer: 10mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2026 Jan 22 RgGuinier 6.4 nm Dmax 22.5 nm VolumePorod 365 nm3

SASDZ24 – Plasminogen R561A point mutant Sample: Plasminogen R561A - pointmutant monomer, 88 kDa Homo sapiens, production … protein Buffer: 10mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2026 Jan 13 RgGuinier 3.7 nm Dmax 12.4 nm VolumePorod 162 nm3

SASDZ34 – Plasminogen (CAT) S741A point mutant Sample: Plasminogen S741A - point mutant monomer, 88 kDa Homo sapiens, production … protein Buffer: 10mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2026 Jan 13 RgGuinier 4.2 nm Dmax 13.0 nm VolumePorod 201 nm3