Capturing transient states of heterodimeric ABC transporter TM287/288 by Time-Resolved Small-Angle X-ray Scattering.

Schröder L, De Vecchis D, Gruzinov A, Schäfer LV, Blanchet CE, Seeger MA, Tidow H, Josts I
Biophys J (2026 Apr 18)

PMID: 42001237 doi: 10.1016/j.bpj.2026.04.016

Submitted to SASBDB: 2026 Mar 26 Published in SASBDB: 2026 Apr 17

SASDZ64 – ABC transporter TM287/288 - heterodimer Sample: ABC transporter TM288 subunit monomer, 68 kDa Thermotoga maritima (strain … protein ABC transporter, ATP-binding protein monomer, 64 kDa ABC transporter TM287 … protein Buffer: 20 mM HEPES, 200 mM NaCl, 5 mM MgCl2, 20 μM DMM, 1 mM Mg2+ ATP, pH: 7.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Sep 10 RgGuinier 5.1 nm

SASDZ74 – ABC transporter TM287/288 heterodimer in complex with nanobody Nb_TM#1 Sample: ABC transporter TM288 subunit monomer, 68 kDa Thermotoga maritima (strain … protein ABC transporter, ATP-binding protein monomer, 64 kDa ABC transporter TM287 … protein Nanobody Nb_TM#1 bound to TM287/288 ABC transporter monomer, 14 kDa Vicugna pacos (alpaca) Buffer: 20 mM HEPES, 200 mM NaCl, 5 mM MgCl2, 20 μM DMM, 1 mM Mg2+ ATP, pH: 7.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Sep 10 RgGuinier 5.4 nm

SASDZ84 – ABC transporter TM287/288 heterodimer in presence of Sybody Sb#35 Sample: ABC transporter TM288 subunit monomer, 68 kDa Thermotoga maritima (strain … protein ABC transporter, ATP-binding protein monomer, 64 kDa ABC transporter TM287 … protein Synthetic nanobody Sb_TM#35 bound to TM287/288 ABC transporter monomer, 14 kDa synthetic (in vitro … Buffer: 20 mM HEPES, 200 mM NaCl, 5 mM MgCl2, 20 μM DMM, 1 mM Mg2+ ATP, pH: 7.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 6 RgGuinier 5.4 nm