A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties

Mardle C, Shakespeare T, Butt L, Goddard L, Gowers D, Atkins H, Vincent H, Callaghan A
Scientific Reports 9(1) (2019 Dec)

doi: 10.1038/s41598-019-44385-y
Submitted to SASBDB: 2018 Jul 30
Published in SASBDB:

SASDE52 – Ribonuclease E from Escherichia coli

Endoribonuclease E experimental SAS data
DAMFILT model
Sample: Endoribonuclease E tetramer, 247 kDa Escherichia coli protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
RgGuinier 5.0 nm
Dmax 16.1 nm
VolumePorod 468 nm3

SASDE62 – Ribonuclease E from Yersinia pestis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 248 kDa Yersinia pestis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
RgGuinier 5.1 nm
Dmax 16.4 nm
VolumePorod 470 nm3

SASDE72 – Ribonuclease E from Francisella tularensis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 256 kDa Francisella tularensis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
RgGuinier 5.1 nm
Dmax 17.2 nm
VolumePorod 491 nm3

SASDE82 – Ribonuclease E from Burkholderia pseudomallei

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 250 kDa Burkholderia pseudomallei protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
RgGuinier 4.8 nm
Dmax 14.9 nm
VolumePorod 437 nm3

SASDE92 – Ribonuclease E from Acinetobacter baumannii

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 254 kDa Acinetobacter baumannii protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
RgGuinier 5.2 nm
Dmax 18.3 nm
VolumePorod 508 nm3