A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties.

Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
Sci Rep 9(1):7952 (2019 May 28)

PMID: 31138855 doi: 10.1038/s41598-019-44385-y

Submitted to SASBDB: 2018 Jul 30 Published in SASBDB: 2019 May 28

SASDE52 – Ribonuclease E from Escherichia coli Sample: Endoribonuclease E tetramer, 247 kDa Escherichia coli protein Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11 RgGuinier 5.0 nm Dmax 16.1 nm VolumePorod 468 nm3

SASDE62 – Ribonuclease E from Yersinia pestis Sample: Endoribonuclease E tetramer, 248 kDa Yersinia pestis protein Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11 RgGuinier 5.1 nm Dmax 16.4 nm VolumePorod 470 nm3

SASDE72 – Ribonuclease E from Francisella tularensis Sample: Endoribonuclease E tetramer, 256 kDa Francisella tularensis protein Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11 RgGuinier 5.1 nm Dmax 17.2 nm VolumePorod 491 nm3

SASDE82 – Ribonuclease E from Burkholderia pseudomallei Sample: Endoribonuclease E tetramer, 250 kDa Burkholderia pseudomallei protein Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11 RgGuinier 4.8 nm Dmax 14.9 nm VolumePorod 437 nm3

SASDE92 – Ribonuclease E from Acinetobacter baumannii Sample: Endoribonuclease E tetramer, 254 kDa Acinetobacter baumannii protein Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11 RgGuinier 5.2 nm Dmax 18.3 nm VolumePorod 508 nm3