Engineered variants provide new insight into the structural properties important for activity of the highly dynamic, trimeric protein disulfide isomerase ScsC from Proteus mirabilis

Furlong E, Kurth F, Premkumar L, Whitten A, Martin J
Acta Crystallographica Section D Structural Biology 75(3):296-307 (2019 Mar 1)

doi: 10.1107/S2059798319000081
Submitted to SASBDB: 2018 Sep 18
Published in SASBDB:

SASDEK4 – C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein (ScsC)

C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein experimental SAS data
DAMFILT model
Sample: C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein monomer, 20 kDa Proteus mirabilis ATCC ... protein
Buffer: 10mM HEPES, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 5
RgGuinier 1.7 nm
Dmax 5.4 nm
VolumePorod 22200 nm3

SASDER4 – Wild-type suppressor of copper sensitivity C protein, PmScsC, with concentration series data

Suppressor of Copper Sensitivity C protein experimental SAS data
OTHER model
Sample: Suppressor of Copper Sensitivity C protein trimer, 74 kDa Proteus mirabilis protein
Buffer: 10mM HEPES, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 5
RgGuinier 3.7 nm
Dmax 11.1 nm
VolumePorod 101 nm3

SASDEQ4 – Deletion mutant of the suppressor of copper sensitivity C protein, PmScsC, with concentration series data

Deletion mutant of PmScsC experimental SAS data
PDB model
Sample: Deletion mutant of PmScsC, 23 kDa Proteus mirabilis ATCC ... protein
Buffer: 10mM HEPES, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 5
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 54 nm3