Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor

Caveney N, Pavlin A, Caballero G, Bahun M, Hodnik V, de Castro L, Fornelos N, Butala M, Strynadka N
Structure (2019 May)

doi: 10.1016/j.str.2019.03.019
Submitted to SASBDB: 2018 Nov 23
Published in SASBDB:

SASDEB8 – Bacillus thuringiensis LexA repressor (Bt_LexA)

Bacillus thuringiensis LexA repressor experimental SAS data
CHIMERA model
Sample: Bacillus thuringiensis LexA repressor dimer, 47 kDa Bacillus thuringiensis protein
Buffer: 20 mM Hepes, 300 mM NaCl, 10% glycerol,, pH: 8
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Aug 25
RgGuinier 3.7 nm
VolumePorod 110 nm3

SASDEC8 – Bacillus thuringiensis LexA repressor bound to Bacteriophage pGIL01 gp7 (Bt_LexA_GIL01_gp7)

Bacillus thuringiensis LexA repressorBacteriophage pGIL01 gp7 experimental SAS data
CHIMERA model
Sample: Bacillus thuringiensis LexA repressor dimer, 47 kDa Bacillus thuringiensis protein
Bacteriophage pGIL01 gp7 tetramer, 24 kDa Bacteriophage pGIL01 protein
Buffer: 20 mM Hepes, 300 mM NaCl, 10% glycerol,, pH: 8
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Aug 25
RgGuinier 4.4 nm