Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.

Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
J Struct Biol :107429 (2019 Nov 25)

PMID: 31778770 doi: 10.1016/j.jsb.2019.107429

Submitted to SASBDB: 2019 Jan 30 Published in SASBDB: 2020 Jan 9

SASDEB2 – Cell wall synthesis protein Wag31: Polymer tbWag31 T73E Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15 RgGuinier 19.8 nm Dmax 23.4 nm

SASDEC2 – Cell wall synthesis protein Wag31: Polymer tbWag31 T73A Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15 RgGuinier 21.8 nm Dmax 13.6 nm

SASDFF2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 245-249) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 10.5 nm

SASDFG2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 250-254) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 11.4 nm

SASDFH2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 255-259) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 10.5 nm

SASDFJ2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 10.1 nm

SASDFK2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 265-269) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 9.9 nm

SASDFL2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 9.0 nm

SASDFM2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 275-279) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 9.8 nm

SASDFN2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 280-284) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 9.2 nm

SASDFP2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 285-289) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 8.1 nm

SASDFQ2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 290-294) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 8.1 nm

SASDFR2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 275-279) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 15.2 nm

SASDFS2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 280-284) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 13.2 nm

SASDFT2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 285-289) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 15.3 nm

SASDFU2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 290-294) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 14.5 nm

SASDFV2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 295-299) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 12.1 nm

SASDFW2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 300-304) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 13.1 nm

SASDFX2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 11.1 nm

SASDFY2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 310-314) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 10.6 nm

SASDFZ2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 315-319) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 12.1 nm

SASDF23 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 320-324) Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29 RgGuinier 10.2 nm