Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics.

Plumridge A, Andresen K, Pollack L
J Am Chem Soc (2019 Dec 5)

PMID: 31804813 doi: 10.1021/jacs.9b04461

Submitted to SASBDB: 2019 Jul 9 Published in SASBDB: 2019 Dec 11

SASDFA9 – Poly-adenosine rA30 in 20 mM NaCl Sample: Poly-adenosine monomer, 10 kDa RNA Buffer: 1 mM Na-MOPS, 20 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 2.7 nm Dmax 11.0 nm VolumePorod 17 nm3

SASDFB9 – Poly-adenosine rA30 in 100 mM NaCl Sample: Poly-adenosine monomer, 10 kDa RNA Buffer: 1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 2.4 nm Dmax 10.7 nm VolumePorod 14 nm3

SASDFC9 – Poly-adenosine rA30 in 200 mM NaCl Sample: Poly-adenosine monomer, 10 kDa RNA Buffer: 1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 2.5 nm Dmax 10.5 nm VolumePorod 14 nm3

SASDFD9 – Poly-adenosine rA30 in 400 mM NaCl Sample: Poly-adenosine monomer, 10 kDa RNA Buffer: 1 mM Na-MOPS, 400 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 2.4 nm Dmax 10.0 nm VolumePorod 13 nm3

SASDFE9 – Poly-adenosine rA30 in 600 mM NaCl Sample: Poly-adenosine monomer, 10 kDa RNA Buffer: 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 2.2 nm Dmax 10.2 nm VolumePorod 13 nm3

SASDFF9 – Poly-adenosine rA30 in 1 mM MgCl2 Sample: Poly-adenosine monomer, 10 kDa RNA Buffer: 1 mM Na-MOPS, 20 mM NaCl, 1 mM MgCl2, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Jul 24 RgGuinier 2.5 nm Dmax 10.4 nm VolumePorod 12 nm3

SASDFG9 – Poly-adenosine rA30 in 2 mM MgCl2 Sample: Poly-adenosine monomer, 10 kDa RNA Buffer: 1 mM Na-MOPS, 20 mM NaCl, 2 mM MgCl2, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 2.4 nm Dmax 10.0 nm VolumePorod 12 nm3

SASDFH9 – Poly-adenosine rA30 in 5 mM MgCl2 Sample: Poly-adenosine monomer, 10 kDa RNA Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 2.3 nm Dmax 10.0 nm VolumePorod 15 nm3

SASDFJ9 – Poly-uridine rU30 in 20 mM NaCl Sample: Poly-uridine monomer, 9 kDa RNA Buffer: 1 mM Na-MOPS, 20 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 3.0 nm Dmax 12.0 nm VolumePorod 29 nm3

SASDFK9 – Poly-uridine rU30 in 100 mM NaCl Sample: Poly-uridine monomer, 9 kDa RNA Buffer: 1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 2.7 nm Dmax 12.7 nm VolumePorod 22 nm3

SASDFL9 – Poly-uridine rU30 in 200 mM NaCl Sample: Poly-uridine monomer, 9 kDa RNA Buffer: 1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 2.5 nm Dmax 11.0 nm VolumePorod 15 nm3

SASDFM9 – Poly-uridine rU30 in 400 mM NaCl Sample: Poly-uridine monomer, 9 kDa RNA Buffer: 1 mM Na-MOPS, 400 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 2.3 nm Dmax 10.5 nm VolumePorod 14 nm3

SASDFN9 – Poly-uridine rU30 in 600 mM NaCl Sample: Poly-uridine monomer, 9 kDa RNA Buffer: 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 2.3 nm Dmax 10.0 nm VolumePorod 14 nm3

SASDFP9 – Poly-uridine rU30 in 1 mM MgCl2 Sample: Poly-uridine monomer, 9 kDa RNA Buffer: 1 mM Na-MOPS, 20 mM NaCl, 1 mM MgCl2, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 2.7 nm Dmax 11.0 nm VolumePorod 17 nm3

SASDFQ9 – Poly-uridine rU30 in 2 mM MgCl2 Sample: Poly-uridine monomer, 9 kDa RNA Buffer: 1 mM Na-MOPS, 20 mM NaCl, 2 mM MgCl2, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 2.6 nm Dmax 10.5 nm VolumePorod 16 nm3

SASDFR9 – Poly-uridine rU30 in 5 mM MgCl2 Sample: Poly-uridine monomer, 9 kDa RNA Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 2.5 nm Dmax 10.0 nm VolumePorod 15 nm3

SASDFS9 – Poly-uridine rU30 in 10 mM MgCl2 Sample: Poly-uridine monomer, 9 kDa RNA Buffer: 1 mM Na-MOPS, 20 mM NaCl, 10 mM MgCl2, 20 µM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 2.3 nm Dmax 9.5 nm VolumePorod 14 nm3