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18 hits found for Caria

SASDC63 – Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide (FPV039:BAK)

Bcl-2-like protein FPV039Uncharacterized protein (BAK1) experimental SAS data
Bcl-2-like protein FPV039 Uncharacterized protein (BAK1) Kratky plot
Sample: Bcl-2-like protein FPV039 monomer, 17 kDa Fowlpox virus protein
Uncharacterized protein (BAK1) monomer, 3 kDa Gallus gallus protein
Buffer: 20 mM trisodium citrate pH, 200 mM NaCl, pH: 6
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Oct 2
Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039. J Biol Chem 292(22):9010-9021 (2017)
...Caria S, Skinner MA, Kvansakul M
RgGuinier 2.0 nm

SASDFK3 – Splicing factor, proline- and glutamine-rich (SFPQ)

Splicing factor, proline- and glutamine-rich experimental SAS data
CORAL model
Sample: Splicing factor, proline- and glutamine-rich dimer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 250 mM NaCl, 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 19
A new crystal structure and small-angle X-ray scattering analysis of the homodimer of human SFPQ. Acta Crystallogr F Struct Biol Commun 75(Pt 6):439-449 (2019)
...Caria S, Lee M
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 91 nm3

SASDBV4 – Vaccinia virus MVA F1L antiapoptotic Bcl-2 viral protein

MVA F1L antiapoptotic Bcl-2 viral protein experimental SAS data
BUNCH model
Sample: MVA F1L antiapoptotic Bcl-2 viral protein dimer, 51 kDa Vaccinia virus protein
Buffer: 25 mM HEPES 150 mM NaCl 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 15
The N Terminus of the Vaccinia Virus Protein F1L Is an Intrinsically Unstructured Region That Is Not Involved in Apoptosis Regulation. J Biol Chem 291(28):14600-8 (2016)
Caria S, Marshall B, Burton RL, Campbell S, Pantaki-Eimany D, Hawkins CJ, Barry M, Kvansakul M
RgGuinier 3.4 nm
Dmax 16.2 nm
VolumePorod 74 nm3

SASDAD8 – Antiapoptotic membrane protein, (DpV84gp022) Deerpox virus

Antiapoptotic membrane protein experimental SAS data
Antiapoptotic membrane protein Kratky plot
Sample: Antiapoptotic membrane protein dimer, 39 kDa Deerpox virus W-1170-84 protein
Buffer: 25 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2013 May 4
Structural basis of Deerpox virus-mediated inhibition of apoptosis. Acta Crystallogr D Biol Crystallogr 71(Pt 8):1593-603 (2015)
...Caria S, Marshall B, Barry M, Kvansakul M
RgGuinier 2.6 nm
Dmax 11.1 nm
VolumePorod 61 nm3

SASDMG8 – Splicing factor, proline- and glutamine-rich-QM bound to Non-POU domain-containing octamer-binding protein: SFPQ(276-598)-QM / NONO(53-312) heterodimer

Non-POU domain-containing octamer-binding proteinSplicing factor, proline- and glutamine-rich experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Non-POU domain-containing octamer-binding protein monomer, 30 kDa Homo sapiens protein
Splicing factor, proline- and glutamine-rich monomer, 38 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 5
Structural basis of the zinc-induced cytoplasmic aggregation of the RNA-binding protein SFPQ Nucleic Acids Research 48(6):3356-3365 (2020)
...Caria S, Lim Y, Badhan R, Anggono V, Lee M
RgGuinier 4.0 nm
Dmax 16.5 nm
VolumePorod 90 nm3

SASDMH8 – Splicing factor, proline- and glutamine-rich-QM-TM bound to Non-POU domain-containing octamer-binding protein: SFPQ(276-598)-QM-TM / NONO(52-312) heterodimer

Splicing factor, proline- and glutamine-richNon-POU domain-containing octamer-binding protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Splicing factor, proline- and glutamine-rich monomer, 38 kDa Homo sapiens protein
Non-POU domain-containing octamer-binding protein monomer, 30 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 8
Structural basis of the zinc-induced cytoplasmic aggregation of the RNA-binding protein SFPQ Nucleic Acids Research 48(6):3356-3365 (2020)
...Caria S, Lim Y, Badhan R, Anggono V, Lee M
RgGuinier 3.6 nm
Dmax 16.5 nm
VolumePorod 80 nm3

SASDUL9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 11.6 nm
VolumePorod 23 nm3

SASDUM9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 13.8 nm
VolumePorod 18 nm3

SASDUQ9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.7 nm
Dmax 11.8 nm
VolumePorod 22 nm3

SASDUP9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.0 nm
Dmax 14.4 nm
VolumePorod 31 nm3

SASDUN9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 29
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.7 nm
Dmax 11.9 nm
VolumePorod 23 nm3

SASDUR9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.0 nm
Dmax 12.3 nm
VolumePorod 28 nm3

SASDUS9 – Wild-type Elastin-like Polypeptide (ELP) in 0.5 M Urea

Elastin-like polypeptide experimental SAS data
Elastin-like polypeptide Kratky plot
Sample: Elastin-like polypeptide monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 10.7 nm
VolumePorod 47 nm3

SASDUT9 – Br-labelled Elastin-like Polypeptide (ELP) in 0.5 M Urea

Elastin-like polypeptide labeled with unnatural amino acid diBrK experimental SAS data
Elastin-like polypeptide labeled with unnatural amino acid diBrK Kratky plot
Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 18.4 nm
VolumePorod 43 nm3

SASDUV9 – Wild-type Elastin-like Polypeptide (ELP) in 2 M Urea

Elastin-like polypeptide experimental SAS data
Elastin-like polypeptide Kratky plot
Sample: Elastin-like polypeptide monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.5 nm
Dmax 18.0 nm
VolumePorod 53 nm3

SASDUU9 – Br-labelled Elastin-like Polypeptide (ELP) in 2 M Urea

Elastin-like polypeptide labeled with unnatural amino acid diBrK experimental SAS data
Elastin-like polypeptide labeled with unnatural amino acid diBrK Kratky plot
Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.5 nm
Dmax 14.8 nm
VolumePorod 48 nm3

SASDUW9 – Wild-type Elastin-like Polypeptide (ELP) in 6 M Urea

Elastin-like polypeptide experimental SAS data
Elastin-like polypeptide Kratky plot
Sample: Elastin-like polypeptide monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.7 nm
Dmax 21.7 nm
VolumePorod 52 nm3

SASDUX9 – Br-labelled Elastin-like Polypeptide (ELP) in 6 M Urea

Elastin-like polypeptide labeled with unnatural amino acid diBrK experimental SAS data
Elastin-like polypeptide labeled with unnatural amino acid diBrK Kratky plot
Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.8 nm
Dmax 18.1 nm
VolumePorod 47 nm3