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5 hits found for Crespo

SASDX82 – N-terminal domain of relaxase from Bacillus subtilis conjugative plasmid pLS20

Relaxase experimental SAS data
PHENIX model
Sample: Relaxase monomer, 29 kDa Bacillus subtilis subsp. … protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
Structure of relaxase from Bacillus subtilis conjugative plasmid pLS20 reveals a new mechanism of DNA processing mediated by dimerization
Isidro Crespo
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 48 nm3

SASDX92 – N-terminal domain of relaxase from Bacillus subtilis conjugative plasmid pLS20 in complex with nic sequence DNA

Relaxasenic sequence, single stranded experimental SAS data
PHENIX model
Sample: Relaxase dimer, 57 kDa Bacillus subtilis subsp. … protein
nic sequence, single stranded dimer, 12 kDa Bacillus subtilis DNA
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
Structure of relaxase from Bacillus subtilis conjugative plasmid pLS20 reveals a new mechanism of DNA processing mediated by dimerization
Isidro Crespo
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 50 nm3

SASDVT5 – Marchantia polymorpha Auxin Response Factor 3

Auxin response factor experimental SAS data
GASBOR model
Sample: Auxin response factor monomer, 46 kDa Marchantia polymorpha protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1. Structure (2025)
Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR
RgGuinier 2.6 nm
Dmax 6.2 nm
VolumePorod 59 nm3

SASDVU5 – Marchantia polymorpha Auxin Response Factor 3 in complex with high affinity DNA

Auxin response factorHigh Affinity ARF binding sequence inverted repeat with 6 nucleotide spacing experimental SAS data
SWISSMODEL model
Sample: Auxin response factor monomer, 46 kDa Marchantia polymorpha protein
High Affinity ARF binding sequence inverted repeat with 6 nucleotide spacing dimer, 12 kDa DNA
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1. Structure (2025)
Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR
RgGuinier 3.1 nm
Dmax 8.4 nm
VolumePorod 67 nm3

SASDVV5 – Arabidopsis thaliana Auxin Response Factor 1 in complex with high affinity DNA

Auxin response factor 1High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing experimental SAS data
Auxin response factor 1 High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing Kratky plot
Sample: Auxin response factor 1 dimer, 81 kDa Arabidopsis thaliana protein
High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing dimer, 13 kDa DNA
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1. Structure (2025)
Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR
RgGuinier 3.6 nm
Dmax 10.5 nm
VolumePorod 136 nm3