Small Angle Scattering Biological Data Bank SASBDB


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5 hits found for Ekal

SASDX84 – Peroxisomal targeting signal receptor Pex5 from P. pastoris

Peroxisomal targeting signal receptor experimental SAS data

Sample:	Peroxisomal targeting signal receptor monomer, 65 kDa Komagataella pastoris protein
Buffer:	50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 May 15

Structure of Pex8 in complex with peroxisomal receptor Pex5 reveals its essential role in peroxisomal cargo translocation (2025)
Ekal L, Wendscheck D, David Y, Chojnowski G, Jeffries C, Mullapudi E, Schuldiner M, Warscheid B, Zalckvar E, Wilmanns M

R_g^Guinier	5.0	nm
D_max	21.4	nm
Volume^Porod	131	nm³

SASDX94 – Peroxisomal biogenesis factor 8 (Pex8) from P. pastoris

Peroxisomal biogenesis factor 8 experimental SAS data

Sample:	Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein
Buffer:	50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17

R_g^Guinier	3.6	nm
D_max	13.0	nm
Volume^Porod	117	nm³

SASDXA4 – Peroxisomal biogenesis factor 8 (Pex8) bound to Peroxisomal targeting signal receptor, Pex5 from P. pastoris

Peroxisomal targeting signal receptorPeroxisomal biogenesis factor 8 experimental SAS data

Sample:	Peroxisomal targeting signal receptor monomer, 65 kDa Komagataella pastoris protein Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein
Buffer:	50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17

R_g^Guinier	5.1	nm
D_max	23.5	nm
Volume^Porod	247	nm³

SASDXB4 – Peroxisomal biogenesis factor 8 (Pex8) bound to the N-terminal domain of Peroxisomal targeting signal receptor, Pex5 (Pex8-Pex5NTD complex from P. pastoris)

Peroxisomal biogenesis factor 8Peroxisomal targeting signal receptor experimental SAS data

Sample:	Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein Peroxisomal targeting signal receptor monomer, 32 kDa Komagataella pastoris protein
Buffer:	50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17

R_g^Guinier	4.6	nm
D_max	19.0	nm
Volume^Porod	177	nm³

SASDXC4 – Peroxisomal biogenesis factor 8 (Pex8) bound to the C-terminal domain of Peroxisomal targeting signal receptor, Pex5 (Pex8-Pex5CTD complex from P. pastoris)

Sample:	Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein Peroxisomal targeting signal receptor monomer, 36 kDa Komagataella pastoris protein
Buffer:	50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17

R_g^Guinier	4.2	nm
D_max	14.5	nm
Volume^Porod	141	nm³

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SASDX84 – Peroxisomal targeting signal receptor Pex5 from P. pastoris

SASDX94 – Peroxisomal biogenesis factor 8 (Pex8) from P. pastoris

SASDXA4 – Peroxisomal biogenesis factor 8 (Pex8) bound to Peroxisomal targeting signal receptor, Pex5 from P. pastoris

SASDXB4 – Peroxisomal biogenesis factor 8 (Pex8) bound to the N-terminal domain of Peroxisomal targeting signal receptor, Pex5 (Pex8-Pex5NTD complex from P. pastoris)

SASDXC4 – Peroxisomal biogenesis factor 8 (Pex8) bound to the C-terminal domain of Peroxisomal targeting signal receptor, Pex5 (Pex8-Pex5CTD complex from P. pastoris)